AP Biology Colony Transformation Lab

What is genetic transformation?

• A prokaryotic cell turning into a eukaryotic cell

• Foreign DNA being inserted into a plasmid

• A host organism taking in foreign DNA

• A gene being mutated

What is the purpose of the Luria broth (LB) when performing genetic transformation?

• Provides resistance to antibiotics

• Provides nutrients needed for bacteria growth

• Stabilizes the plasmid being given to the host organism

• Sterilizes the instruments

Which is not a characteristic of prokaryotic cells?

• contains a nucleus

• contains plasmid DNA

• single-celled

• enclosed by a cell wall

A "-amp^R" cell

• has the amp^R gene in it's plasmid and is resistant to ampicillin

• does not have the amp^R gene in it's plasmid and is resistant to ampicillin

• has the amp^R gene in it's plasmid and is not resistant to ampicillin

• does not have the amp^R gene in it's plasmid and is not resistant to ampicillin

What does it mean for cells to be competent

• They are made to be 'smart' through genetic engineering

• Their cell walls are altered to allow DNA to pass more easily

• They contain regular DNA and plasmid DNA

• They contain a gene for resistance to ampicillin

What process increases the competency of cells?

• calcium chloride and an ice bath

• calcium chloride and heat shock

• Luria broth (LB) and an ice bath

• Luria broth (LB) and heat shock

Which is the correct sequence for the process of genetic transformation?

• bacterial cells transferred to cold CaCl₂ , plasma with foreign DNA added bacteria , cells heat shocked at 42^oC , cells spread on agar petri dish , bacteria given time to grow on petri dish

• bacterial cells transferred to cold CaCl₂ , cells heat shocked at 42^oC , plasma with foreign DNA added bacteria , bacteria given time to grow on petri dish , cells spread on agar petri dish

• plasma with foreign DNA added bacteria , bacterial cells transferred to cold CaCl₂ , cells spread on agar petri dish , bacteria given time to grow on petri dish , cells heat shocked at 42^oC

Which one of these agar plates contains bacteria most like the original bacterial culture

• +pGLO LB/amp

• +pGLO LB/amp/ara

• -pGLO LB/amp

• -pGLO LB

Which one of these agar plates would there be no bacteria growth?

• +pGLO LB/amp

• +pGLO LB/amp/ara

• -pGLO LB/amp

• -pGLO LB

Which image is a plasmid?

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