Genetic Engineering and Gel Electrophoresis

In preparation for an electrophoresis procedure, enzymes are added to DNA in order to

• convert the DNA into gel

• cut the DNA into fragments

• change the color of the DNA

• produce longer sections of DNA

the electrode that the DNA migrates towards is:

• positive

• negative

When separating genetic material using gel electrophoresis, the only factor that determines the distance a fragment migrates is size. Is this statement correct?

• no, the specific sequence of nucleotides also determines the distance it migrates in the gel

• no, DNA fragments of the same length will travel differently depending on the charge of the DNA

• yes, because all DNA molecules are negatively charged, so changing the fragment size changes the mass tho charge ratio proportionally

Your lab partner made an agarose gel with the wells in the center and loaded unknown molecules in the gel. After performing gel electrophoresis, you found that your molecules migrated to position A, B, and C in the image, respectively. What conclusion can you draw from these results?

• the molecules in position A are positively charged, the ones in B are neutral, and the ones in C are negatively charged

• the molecules in position A are likely DNA, the ones in position C are RNA

• the molecules in position A are larger than those in positions B and C

If you changed the agarose gel concentration, how would your results be impacted?

• the molecules would travel in a different direction

• the molecules would travel at a different speed

• the molecules would travel a different relative distance

Given the following results, who is the father of the child in question?

• Dad 1

• Dad 2

• Dad 3

Tags

NGSS.HS-LS3-1

NGSS.HS-LS3-2