Restriction Enzyme Cuts

Enzymes are an example of

• Lipids

• Carbohydrate

• Protein

• Nucleic Acid

Tags

NGSS.HS-LS1-1

In order to insert a human gene into plasmid, both must

• code for the same gene product.

• be cut by the same restriction enzyme.

• originate from the same type of cell.

• have identical sequences.

The site where restriction enzymes cut are a called:

• cut sites

• restriction sites

• recognition sites

• cleave sites

How many times do you want a restriction enzyme to cut a human genome in order to cut out the gene of interest to insert into a plasmid?

• 1 - through the gene

• 2 - on either side of the gene

• 3 - through the gene and on either side of the gene

• 4 - twice on each side of the gene

Which is the primary purpose of using restriction enzymes in gel elctrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing
• It restricts the number of base pairs that can be tested in a sample
• It makes the testing simpler by moving the strands into the gel faster
• It charges the DNA strands

Asymmetrical ends of DNA after being cut by a restriction enzyme

• Blunt Ends

• Sticky Ends

• Ligated Ends

• Restrictive Ends

Enzymes used to cut DNA into pieces at specific points

• Restriction enzymes

• Recombinant DNA

• Genome

• Plasmid

In bacteria, restriction enzymes are used for defense. They cut up the DNA of a virus that infects bacteria.

• True
• False