Recombinant DNA Technology

The plasmids carrying the target gene (recombinant plasmids) must be introduced into a host cell through _____.

• Cloning

• insertion

• Transformation

• Isolation

Types of plasmids produced after insertion of the target gene into a vector.

• Recombinant plasmids

• Non-recombinant plasmids

• Bacterial plasmids

• Isolated plasmids

Which technology below would probably be the most important to a person who had diabetes and had to take insulin every day?

• using recombinant DNA to produce human hormones from bacteria

• testing parents for genetic disorders before they have children

• engineering fruits and vegetables that resist insects and other pests

• C. developing ways to identify criminals through DNA fingerprinting

In order to insert a human gene into plasmid, both must

• code for the same gene product.

• be cut by the same restriction enzyme.

• originate from the same type of cell.

• have identical sequences.

DNA is created by using the ______ from a bacteria and splicing a section of DNA into it.

• recombinant, plasmid

• Cloning, ribosome

• transcription, translation

• cumulus, mitochondria

Which of the following is NOT a tool used in recombinant DNA technology?

• Cloning vector

• RNA polymerase

• Target DNA

• DNA ligase

Recombinant DNA technology involves a screening process. What is the purpose of screening process?

• Isolate recombinant DNA from bacteria

• Identify cell with target DNA to be cut by restriction enzyme

• Identify bacteria with plasmid to be used as vector

• Identify bacteria with recombinant plasmid

First stage involved in gene cloning.

• Amplification of the target gene

• Insertion of the target gene

• Polymerase Chain Reaction

• Isolation of the target gene

How do scientists cut DNA into smaller strands?

• restriction enzymes

• scissors

• ligase

• agarose

Which enzyme "glues" DNA fragments together during insertion?

• restriction enzymes

• DNA ligase

• DNA polymerase

• DNA helicase