Unit 6 AP Bio

What is the enzymatic function of restriction enzymes?

• to cleave nucleic acids at specific sites

• to add new nucleotides to the growing strand of DNA

The segment of DNA shown in the figure has restriction sites I and II, which create restriction fragments A, B, and C. Which of the gels produced by electrophoresis shown below best represents the separation and identity of these fragments?

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Tags

NGSS.HS-LS3-1

Which of the following are characteristics of Prokaryotic DNA? (more than 1 answer applies)

• Single linear strand

• Single circular strand

• Multiple linear strands

• Plasmids

• Contains histones

Which of the following describe the structure of mature RNA? (more than 1 answer applies)

• Contains Uracil

• Contains introns and exons

• Has a poly-A tail and 5' cap

• Single stranded

• Circular strand

Which of the following would be transcribed from the following DNA sequence? 3' ATC GAA TTA CGA 5'

• 5' UAG CUU AAU GCU 3'

• 5' AUC GAA UUA CGA 3'

• 3' UCG UAA UUC UAG 5'

• 3' TAG CTT AAT GCT 5'

• 5' TAG CTT AAT GCT 3'

Tags

NGSS.HS-LS1-1

Which of the following is true of DNA Replication?

• DNA is semi conservative because it creates two new strands of old nucleotide.

• New DNA strands are being synthesized in the 5' to 3' direction

• DNA is being synthesized in the 3' to 5' directions from a template strand

• RNA is being synthesized by RNA polymerase in the 5' to 3' direction

• A sense strand is created from a nonsense strand of DNA using RNA polymerase

Where does RNA polymerase bind to DNA?

• at an activator site

• on the coding gene sequence

• at a promoter region

• downstream of the coding sequence

• Where the RNA primer is placed

Which of the following occur during RNA processing? (more than 1 answer applies)

• exons can be removed

• spliceosomes remove introns

• a poly-A tail and GTP cap are added

• DNA helicase unzips DNA

• activator sequences initiate transcription

Which of the following is a similarity between the process prokaryotic and eukaryotic organisms implement for protein synthesis?

• Transcription takes place in the nucleus, translation takes place in the cytoplasm

• Introns are removed by spliceosomes

• RNA processing adds a cap and tail to the mature RNA strand

• Transcription and translation happen simultaneously

• Ribosomes for phosphodiester bonds between amino acids

Bacterial cells that contain pGal will fluoresce blue under ultraviolet light. Which of the following is the most likely outcome of replacing the lacA gene in the E. coli lac operon with the gene encoding pGal?

• When lacY and lac Z are breaking down and transporting lactose, the bacteria will fluoresce blue under ultraviolet light

• lacZ will only produce the enzyme for breaking down galactose in the presence of ultraviolet light

• the repressor enzyme will only be able to bind to the operator under the presence of lactose

• When the regulatory gene is stimulated by lactose, it will cause the gene to fluoresce blue

• All structural genes downstream from the placement of lacA will be inhibited due to this substitution mutation.

Tags

NGSS.HS-LS1-1

NGSS.HS-LS3-2