Mi 4.3

How is DNA synthesis in PCR and Cycle Sequencing the SAME?

• Both require DNA polymerase.

• Both require primers.

• Both require deoxynucleotides (dNTPs).

• Both require dideoxynucleotides (dNTPs).

• Both generate new DNA products of equal length.

DNA has a ___________ charge and will migrate through a gel towards the ___________ electrode.

• positive, negative

• positive, positive

• negative, positive

• negative, negative

What do dideoxynucleotides do when added to a growing DNA strand during Cycle Sequencing?

• They stop the elongation of that strand.

• That double the length of that strand.

• They shorten the length of the strand.

• They denature the strand.

Which of the following base pairs is matched correctly?

• Guanine and Thymine

• Cytosine and Adenine

• Adenine and Thymine

• Thymine and Cytosine

How is DNA synthesis in PCR and Cycle Sequencing DIFFERENT?

• Only PCR uses deoxynucleotides.

• Cycle Sequencing generates DNA products of different lengths.

• Only PCR analyzes DNA products using gel electrophoresis.

• Only Cycle Sequencing uses dideoxynucleotides.

Larger DNA fragments move through a gel _____________ smaller DNA fragments.

• faster than

• at the same rate as

• slower than

Place the steps of DNA Sequencing below into their correct order.

• A, B, C, D

• B, C, A, D

• B, A, C, D

• D, B, C, A

What is the primary function of the Mi 4.3 device in scientific research?

• Data collection

• Data analysis

• Data storage

• Data visualization

Which of the following components is essential for the operation of Mi 4.3 in a laboratory setting?

• Power supply

• Cooling system

• Optical sensor

• Magnetic field

Tags

NGSS.HS-PS2-5

In an experiment using Mi 4.3, which variable is most likely to be measured?

• Temperature

• Pressure

• Light intensity

• Sound frequency

Tags

NGSS.MS-PS1-4

NGSS.MS-PS3-4