Column Chromatography

Ava, Oscar, and Freya are conducting a science experiment. They observe that in the stationary phase of their experiment, certain characteristics of molecules come into play. Which statement is TRUE about this phase?

The ____ volatile molecules move ____ in the stationary phase.

• less, faster

• more, faster

• less, slower

• more, slower

Answer explanation

In the stationary phase of the experiment, less volatile molecules move slower. This is because less volatile molecules have a lower tendency to evaporate and a higher affinity for the stationary phase, causing them to move at a slower pace compared to more volatile molecules.

The time required for a component to elute out of the column is called what?

• retention time

• elution time

• column time

• reaction time

An analyte with a (low/high) chemical affinity with the column will elute quickly through the column

In column chromatography, lysed cells are added to the column. The cellular ​ (a)   move through a semi-solid (b)   that separates them. The matrix is held up in the column by the ​ (c)   , a porous disk at the bottom of the column. ​Liquid (d)   is run through to carry proteins down and out of the column.

• matrix

• frit

• buffer

• proteins

• GFP

• lipids

• water

Which of the following is true of column chromatography in this lab? (Choose all that are true.)

• larger proteins will elute first

• smaller proteins will elute last

• smaller proteins will elute first

• larger proteins will elute last

A protein is said to ________ when is moves through and out of the chromatography column.

Types according to the force of separation of Chromatography...

• Adsorption chromatography

• Partition chromatography.

• Column chromatography

• Thin layer chromatography

What is the primary purpose of column chromatography in scientific experiments?

• To separate components of a mixture

• To measure the pH of a solution

• To determine the boiling point of a liquid

• To calculate the density of a solid

In column chromatography, what is the role of the stationary phase?

• To move the sample through the column

• To separate components based on their affinity

• To provide a color indicator

• To act as a catalyst

Which of the following is a common stationary phase material used in column chromatography?

• Silica gel

• Water

• Ethanol

• Sodium chloride