Gel Electrophoresis Quiz

What is Electrophoresis?

• A technique used to separate nucleic acids (DNA / RNA) and proteins according to size.

• A method for measuring the temperature of liquids.

• A process for purifying water.

• A technique for analyzing gas mixtures.

What factors influence the migration of molecules in electrophoresis?

• The applied electric field and the rigid, mazelike matrix of the gel support.

• The temperature of the gel.

• The color of the dye used.

• The size of the electrophoresis apparatus.

What is the purpose of staining nucleic acids in gel electrophoresis?

• To visualize DNA.

• To increase the temperature of the gel.

• To change the pH of the solution.

• To enhance the electric current.

What is the effect of molecular mass on DNA migration?

• Small DNA pieces move faster than larger ones.

• Larger DNA pieces move faster than smaller ones.

• All DNA pieces move at the same speed.

• DNA migration is not affected by size.

What are the two types of protein electrophoresis?

• 1D and 2D protein electrophoresis.

• SDS-PAGE and agarose gel electrophoresis.

• Gas chromatography and liquid chromatography.

• Capillary electrophoresis and gel filtration.

What is the role of SDS in 1D protein electrophoresis?

• To coat polypeptides with negative charges.

• To increase the size of proteins.

• To denature the proteins.

• To enhance the visibility of proteins.

What is the purpose of using pre-stained protein ladders in SDS-PAGE?

What does the isoelectric point of a protein indicate?

What are the two steps involved in 2D Gel Electrophoresis?

What is the significance of restriction mapping in plasmid construction?