W25 Tutorial 3 Section 023

The human genome is 3

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As a biologist, you recently discovered a new organism. This is likely an eukaryotic organism.

• This is likely an eukaryotic organism

• It is likely that a mitochondrion is present.

Determine the described transposable element: Using a eukaryotic organism such as yeast, a fragment of DNA containing intron is inserted into the transposable element. After transposition, the entire fragment of the DNA is present at another location in the genome without the intron, and the DNA fragment is missing from the original site, with only long terminal repeats left.

• The described transposable element is a retrotransposon

• The described transposable element is a DNA transposon

The TRA gene in Drosophila is translated in females but not in males. Both sexes have an SXL binding site, and if an SXL protein is present, it can bind and push the splicing mechanism U2AF to a 3' splice site further down in the transcript. Which of the following options is TRUE? Multiple Choice.

• SXL protein is present

• SXL protein is present in females only

What of the following would occur if the lac super repressor was on one of two plasmids in a cell? Multiple Choice.

• The lac repressor will suppress the lac operon

• The lac repressor will repress the lac operon

What is the minimum amount of bases required in a sequence that we can expect to be unique in this database?

• 83

• 10000

Which of the following spontaneous mutations would most likely NOT result in Indels (insertions/deletions)?

• strand slippage

• intercalating agents

A researcher is studying a gene involved in neural development. They generate a heterozygous knockout mouse carrying one functional allele (WT) and one null allele (-). Surprisingly, the mouse exhibits severe neurological defects similar to those observed in homozygous knockouts. To confirm whether this gene is haploinsufficient, the researcher introduces a third copy of the functional allele (WT) into heterozygous mice. Which of the following outcomes would most strongly support haploinsufficiency?

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The expression of genes: Information about the pI. It is likely that this species is?

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The described transposon: The described transposon. No such transposon exists.

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• 1