Application of Recombinant DNA Technology : Insulin

What is a cDNA?

• collection of total genomic DNA

• non-coding RNA molecule (containing about 22 nucleotides) found in plants,

• complementary DNA molecules synthesized from mRNA

• small interfering Ribonucleic Acid, is a class of double-stranded RNA molecules

Which of the following is/are true about reverse transcriptase

• works backwards, from DNA to RNA

• works forward from RNA to DNA

• works backwards from RNA to DNA

• works forward from DNA to RNA

the function of reverse transcriptase is enzyme used to generate complementary DNA from an RNA template

• false

• true

Human insulin produced by genetic engineering is better because (a) it acts faster (b) it lasts longer in the body (c) it does not cause allergic reactions

• a,b, and c
• a and c
• b and c
• a only

The structure from which insulin mRNA can be obtained is ………………………………

• pancreas
• islets of Langerhans
• blood plasma
• liver

in producing insulin by genetic engineering, what is the first step to take?

• isolate insulin from pancrease
• isolate insulin mRNA from pancreas
• isolate insulin DNA from pancreas
• isolate insulin from animal tissue

mRNA is converted to cDNA by ………………

• Ligase enzyme
• Reverse Transcriptase enzyme
• Restriction Endonuclease enzyme
• RNA polymerase enzyme

double stranded DNA is produced by ……………………….

• Ligase enzyme
• Reverse Transcriptase enzyme
• Restriction Endonuclease enzyme
• DNA polymerase enzyme

Why is insulin produced by genetic engineering more rapid in its action?

• it is similar to animal insulin
• It has a shape that is exactly to that of natural human insulin
• It has higher optimum temperature of activity
• it is transported more rapidly by the blood to target sites

What is the name of insulin produced by genetic engineering?

• Humulin G

• Humulin I

• Humulin H

• Humulin S