Biochemistry Quiz

Which mechanism does ATP directly produced in Glycolysis?

• Oxidative phosphorylation

• Substrate level phosphorylation

• Phosphorylation

• Photophosphorylation

Which substrate is used in the last step of glycolysis?

• Pyruvate

• Glyceraldehyde 3-phosphate

• 1, 3-bisphosphoglycerate

• Phosphoenolpyruvate

In absence of oxygen, pyruvate is converted into lactate in muscle because

• during lactate formation, NADH is reconverted into NAD.

• during product of lactate two ATP are produced

• Lactate acts as substrate for formation of amino acid

• Lactate is substrate from downstream pathway

Substrate-level phosphorylation is catalyzed by which of the following enzymes?

• Glycerol kinase

• Galactokinase

• Phosphofructokinase-1

• Pyruvate kinase

• Hexokinase

Which of the following is FALSE about glycogen molecules?

• Glycogen is polysaccharide

• Glycogen is a polymer of beta-D-Glucose

• Glycogen consists of α(1-4) and α (1-6) glycosidic linkage

• Glycogen have are a helical structure with branching.

In glycolysis: 3 irreversible steps are catalyzed by

• pyruvate-kinase (phosphoenolpyruvate to pyruvate)

• hexokinase (glucose to G6P)

• phosphoglycerate kinase (1,3 bisPG to 3PG)

• phosphofructosekinase (F6P to F1,6bisP)

High concentration of glucose 6-phosphate is inhibitory to ___________

• Glucokinase

• Pyruvate kinase

• Hexokinase

• Phosphofructokinase-1

A product of glycolysis that is consumed in alcoholic fermentation is

• CO2

• All of above

• ATP

• NADH

In Bradford protein assay, when Bradford dye reagent binds protein, the dye color will change from?

• Blue to Green

• Blue to Reddish Brown

• Reddish Brown to Blue

• Green to Blue

At which of the below wavelength that absorbance is maximum when Bradford dye reagent binds protein?

• At 460nm

• At 295nm

• At 730nm

• At 595nm