Gene Knockout Techniques Quiz

What is the purpose of a gene knockout in scientific research?

• To enhance the function of a gene

• To make a gene inoperative and study its function

• To duplicate a gene for further study

• To transfer a gene to another organism

Which system is used to silence a gene in gene knockout techniques?

• CRISPR-Cas9

• DNA polymerase

• RNA polymerase

• Ribosome

What is the role of Cas9 in the CRISPR-Cas9 system?

• To replicate DNA

• To bind to RNA

• To excise viral DNA from the genome

• To synthesize proteins

What is the function of guide RNA (gRNA) in the CRISPR-Cas9 system?

• To replicate DNA

• To guide Cas9 to the target DNA

• To synthesize proteins

• To degrade RNA

Which organisms are commonly used as models in gene knockout research due to their genetic similarity to humans?

• Frogs

• Mice

• Birds

• Fish

What is the role of the synthetically derived gRNA in the CRISPR-Cas9 system?

• It acts as a catalyst for DNA replication.

• It binds to a target sequence for excision.

• It provides energy for the Cas9 protein.

• It repairs damaged DNA sequences.

Which of the following is a gene editing application of the CRISPR-Cas9 system in agriculture?

• Increasing the size of fruits.

• Enhancing metabolic pathways for higher starch content.

• Changing the color of flowers.

• Reducing the growth rate of plants.

What is a conserved sequence?

• A sequence that changes rapidly over time.

• A sequence identical across all members of a species.

• A sequence that is unique to each individual.

• A sequence that is only found in extinct species.

Why are highly conserved sequences important in evolutionary studies?

• They show rapid changes in species.

• They help establish phylogenetic relationships.

• They are unique to each species.

• They are only found in modern organisms.

What is one reason for the presence of conserved gene sequences?

• They have a high rate of mutation.

• They serve an essential functional requirement.

• They are not involved in any cellular processes.

• They are only found in plant cells.