Spectroscopy, Chromatography Multiple Choice Questions

What is the dimension of a C18 column 3.5 µm, 4.6 x 150 mm

• The inner diameter is 3.5 µm, the particle size and the length of the column is 4.6 and 150 mm, respectively.

• The particle size is 3.5 µm, the inner diameter and the length of the column is 4.6 and 150 mm, respectively.

• The particle pore is 3.5 µm, the inner diameter and the length of the column is 4.6 and 150 mm, respectively.

• The inner diameter is 3.5 µm, the particle pore and the length of the column is 4.6 and 150 mm, respectively.

Which column is suitable for a HPLC-DAD system?

• A. A C18 BEH (1.7 µm, 2.1 mm x 100 mm)

• B. A C18 BEH (5.0 µm, 4.6 mm x 150 mm)

• C. A capillary column (0.25 µm, 0.25 mm x 30m)

• D. None of the above

The more commonly used chromatographic technique:

• Liquid chromatography

• Gas chromatography

• Thin layer chromatography

• Partition chromatography

Which factor describes how closely the measurements of different days match each other.

• Reproducibility

• Precision

• Accuracy

• Repeatability

Which method is more routinely used for quantitative analysis?

• MALDI-TOF

• UPLC-ESI/MS

In infrared spectroscopy, C-H bonds normally absorb at______than C-C bonds

• Longer wavenumber and higher intensity

• Longer wavenumber and lower intensity

• Shorter wavenumber and higher intensity

• Shorter wavenumber and lower intensity

Which type of ionization source can be more useful for the identification of molecular structure?

• Electrospray ionization

• Electron impact ionization

• Matrix assisted laser desorption ionization

• Atmospheric pressure chemical ionization

What is the most common application of UV-Vis spectroscopy

• To determine the pKa of a molecule

• To be used as a detector for chromatographic techniques

• To separate compounds in a mixture

• To determine the protein concentration

The principle of UV-Vis spectroscopy is based on

• The oscillation of the atoms around the bonds

• The electronic transition of the electrons in the atoms

• The magnetic field created by the spins of the atoms

• The absorption of the light of the molecule

Which type of ionization source can be considered extensive source?

• Electrospray ionization

• Electron impact ionization

• Matrix assisted laser desorption ionization

• Atmospheric pressure chemical ionization