SOLREV10- Biotechnology: Genetic Engineering

Which term best describes a DNA molecule that contains DNA from two different species?

• Recombinant DNA

• GMO

• Gene of interest

• Plasmid transformation

In the recombinant DNA process shown in the diagram, which enzyme is used to cut DNA at restriction sites to open the plasmid and isolate the gene of interest?

• DNA ligase

• RNA polymerase

• Restriction enzymes

• Reverse transcriptase

What is the role of DNA ligase during plasmid transformation as depicted in the diagram?

• It cuts DNA at sticky ends to isolate the gene of interest.

• It seals DNA bonds at sticky ends to produce recombinant DNA.

• It transcribes mRNA from the insulin gene.

• It transports plasmids into bacterial cells.

Which option defines a plasmid in the context of genetic engineering?

• Linear DNA found in animal cells that cannot be manipulated

• Circular DNA found in bacteria that can be manipulated

• Protein complex that transports genes into the nucleus

• Segment of mRNA coding for insulin

A gene that codes for a desired protein, such as insulin, is referred to as which of the following?

• Transgenic sequence

• Gene of interest

• Restriction site

• Vector gene

Tags

NGSS.HS-LS1-1

What term describes an organism that has had its genome altered, potentially through plasmid transformation?

• Transgenic organism

• GMO

• Recombinant DNA

• Mutagen

Which term specifically refers to an organism that contains DNA from another species in its genome?

• Transgenic organism

• GMO

• Gene of interest

• Plasmid transformation

According to the diagram, what biological product is harvested from bacteria after recombinant DNA is produced?

• Antibodies

• Insulin

• Hemoglobin

• Collagen

Tags

NGSS.HS-LS1-1

Identify the primary purpose of restriction enzymes during the genetic engineering process summarized in the diagram.

• To splice mRNA into plasmids

• To cut DNA at specific restriction sites

• To replicate plasmids during cell division

• To translate genes into proteins

Which step logically follows the insertion of the insulin gene into a bacterial plasmid with sticky ends?

• Restriction enzymes cut the plasmid again at random sites.

• DNA ligase seals the bonds to form recombinant DNA.

• The gene of interest is removed to prevent expression.

• The plasmid is converted into linear DNA.

Tags

NGSS.HS-ETS1-3