Pipetting Techniques and Best Practices

This tutorial covers the correct use of a two-button pipette, focusing on proper tip attachment, liquid aspiration, and dispensing techniques. Key factors such as aspiration angle, immersion depth, and dispensing angle are discussed to ensure accurate and reliable pipetting results. The video concludes with tips on tip ejection and maintaining pipette accuracy.

What is the primary function of the control button on a 2-button pipette?

• To calibrate the pipette

• To eject the pipette tip

• To control the aspiration and dispensing of liquid

• To adjust the volume

When attaching the pipette tip, what should you avoid doing?

• Inserting the pipette straight into the tip

• Using force

• Applying light pressure

• Ensuring the tip is secure

What are the two factors that influence accurate liquid uptake?

• Temperature and humidity

• Aspiration angle and immersion depth

• Pipette size and liquid viscosity

• Tip length and pipette brand

What should the aspiration angle be to ensure accurate liquid uptake?

• Vertical

• 20 degrees

• 45 degrees

• Horizontal

Why should the immersion depth be as small as possible?

• To reduce the risk of contamination

• To prevent the pipette from breaking

• To avoid additional liquid uptake due to the capillary effect

• To ensure faster pipetting

What is the first step in aspirating liquid with a pipette?

• Press the control button to the second stop

• Immerse the tip into the liquid

• Release the control button quickly

• Press the control button to the first stop

How many times should you repeat the aspiration process to situate the air in the tip?

• Once

• Two to three times

• Four to five times

• Until the tip is full

At what angle should the pipette be held when dispensing liquid?

• 90 degrees

• 0 to 10 degrees

• 20 to 45 degrees

• 50 to 60 degrees

What ensures that the pipette tip is emptied completely?

• Pressing the control button to the first stop

• Pressing the control button further down to the second stop

• Holding the pipette vertically

• Using a new tip for each aspiration

What is the final step in the pipetting process?

• Calibrating the pipette

• Storing the pipette properly

• Ejecting the tip into a garbage bin

• Cleaning the pipette