Bacterial Cell Transformation and Plating Quiz

The video tutorial guides viewers through the process of bacterial cell transformation and plating. It begins with the preparation of tubes and plates, followed by detailed instructions on how to plate P- and P+ cells using proper aseptic techniques. The tutorial emphasizes careful handling to avoid contamination and concludes with instructions on spreading cells and preparing plates for incubation.

What is the first step after bacterial cell transformation?

• Add LB broth

• Incubate on ice

• Heat shock at 42 degrees

• Label the tubes

How many plates are used for the plating process?

• Four

• Two

• One

• Three

What volume of P- cells is dispensed onto each plate?

• 100 microliters

• 50 microliters

• 25 microliters

• 75 microliters

What should you avoid doing to prevent contamination while handling the pipette tip?

• Touching the top of the pipette

• Mixing the cells too vigorously

• Placing the tip on the bench

• Using a new tip for each plate

What is the correct technique for spreading cells on the plate?

• Using a circular motion

• Using a gentle back and forth motion

• Using a rapid shaking motion

• Using a zigzag motion

How should the P+ cells be mixed before dispensing?

• Vigorously shaking the tube

• Gently mixing up and down

• Stirring with a rod

• Leaving them undisturbed

How much P+ cells are dispensed onto the third plate?

• 100 microliters

• 150 microliters

• 75 microliters

• 50 microliters

What should be done to the plates after spreading the P+ cells?

• Invert and stack them

• Leave them open to dry

• Place them in an incubator

• Cover them with foil

Why is it important to avoid gouging the agar while spreading cells?

• To avoid damaging the cells

• To ensure even distribution

• To speed up the process

• To prevent contamination

How long should the plates sit on the bench before inverting?

• 15 minutes

• 5 minutes

• 2 minutes

• 10 minutes