Kinetics Parameters for Catalase Part 2: kcat and efficiency

The video tutorial explains the calculation of kinetic parameters for the enzyme catalase, including VMAX, KM, Kcat, and catalytic efficiency. It demonstrates how to calculate Kcat using VMAX and enzyme concentration, and discusses the concept of turnover number. The tutorial also covers catalytic efficiency, comparing catalase with carbonic anhydrase, and highlights the importance of unit consistency in calculations.

What is the VMAX value for catalase as mentioned in the video?

• 400,000,000 micromolar per second

• 300,000,000 micromolar per second

• 200,000,000 micromolar per second

• 100,000,000 micromolar per second

How is Kcat related to the enzyme concentration and VMAX?

• Kcat is the ratio of VMAX to enzyme concentration

• Kcat is the difference between enzyme concentration and VMAX

• Kcat is the sum of enzyme concentration and VMAX

• Kcat is the product of enzyme concentration and VMAX

What is the turnover number for catalase?

• 30 million per second

• 50 million per second

• 20 million per second

• 40 million per second

How is catalytic efficiency calculated?

• By multiplying Kcat and KM

• By dividing Kcat by KM

• By dividing KM by Kcat

• By adding Kcat and KM

What is the catalytic efficiency of catalase in inverse micromolar inverse second?

• 1800

• 1600

• 1400

• 1200

Which enzyme has a higher catalytic efficiency, catalase or carbonic anhydrase?

• Cannot be determined

• Catalase

• Carbonic anhydrase

• Both have the same efficiency

What is the KM value for carbonic anhydrase?

• 5 millimolar

• 7 millimolar

• 9 millimolar

• 11 millimolar

Which factor primarily contributes to catalase's higher efficiency compared to carbonic anhydrase?

• Lower Kcat value

• Higher KM value

• Higher Kcat value

• Lower KM value

What does a lower KM value indicate about enzyme binding?

• Tighter binding

• Faster reaction rate

• No effect on binding

• Weaker binding

What is the main measure of how fast an enzyme converts substrate to product?

• KM

• Kcat

• Enzyme concentration

• Catalytic efficiency