Nucleotide Metabolism | Mechanism of OMP (Orotidylate) Decarboxylase

The video tutorial explains the mechanism of OMP decarboxylase, an enzyme that significantly enhances reaction rates by converting rhodolite into uridylate. The enzyme achieves this through a simple yet fascinating mechanism involving charge repulsion and proton transfer. The uncatalyzed reaction would take millions of years, but the enzyme completes it in milliseconds, demonstrating a remarkable rate enhancement.

What is the primary function of the enzyme OMP decarboxylase?

• To convert rhodolite into uridylate

• To synthesize proteins

• To break down carbohydrates

• To enhance photosynthesis

How long does the uncatalyzed reaction of rhodolite to uridylate take?

• 17 milliseconds

• 1 second

• 30 million years

• 10 years

Which residue is critical in the active site of OMP decarboxylase?

• Lysine 93

• Threonine 100

• Histidine 50

• Serine 45

What role does aspartate play in the enzyme's mechanism?

• It binds to the enzyme's active site

• It acts as a proton donor

• It stabilizes the substrate

• It creates charge repulsion with the carboxyl group

What is the result of the charge repulsion in the enzyme's mechanism?

• Formation of a new enzyme

• Decarboxylation and formation of CO2

• Breakdown of proteins

• Synthesis of ATP