The video tutorial explains niacin, also known as vitamin B3, and its role in human metabolism. It covers the synthesis of niacin through the Dinovo pathway and its conversion into active coenzymes NAD and NADP. The tutorial also discusses the convergence of Dinovo and dietary pathways, the salvage pathway for NAD synthesis, and methods for detecting NADH using UV VIS spectrophotometry. An example of lactate dehydrogenase is used to illustrate enzyme activity measurement.

What is the main difference between niacin and other B vitamins regarding its synthesis in humans?

Describe the process by which niacin is transformed into its active forms, NAD and NADP.

What role does quinolinic acid play in the synthesis of niacin?

Explain the significance of nicotinate mononucleotide in the context of dietary niacin.

How does NAD differ from NADH in terms of absorbance at specific wavelengths?

What is the importance of measuring NADH absorbance at 340 nanometers?

Discuss the role of lactate dehydrogenase in the conversion of lactate to pyruvate.