NEW!!! L-Lactate Dehydrogenase: Physiology, Biochemisty, and Mechanism

The video tutorial explores the mechanism of lactate dehydrogenase, an enzyme crucial in anaerobic fermentation. It provides background on glycolysis, the role of NADH, and the impact of oxygen levels on cellular metabolism. The tutorial explains how lactate dehydrogenase helps regenerate NAD+ under low oxygen conditions, allowing glycolysis to continue. The video also details the enzyme's organic mechanism, including the conversion of pyruvate to lactate and vice versa, highlighting the enzyme's role in maintaining cellular energy balance.

Describe the process of glycolysis and its end products.

Explain the significance of NADH in the glycolytic pathway.

What happens to the electron transport chain when oxygen levels are low?

What are the consequences of prolonged anaerobic conditions on cellular metabolism?

What is the relationship between lactate and ATP production during anaerobic respiration?

What is the primary function of lactate dehydrogenase in anaerobic conditions?

Discuss the role of pyruvate in the context of lactate production.

How does lactate dehydrogenase help in regenerating NAD+?

Explain how the structure of lactate dehydrogenase facilitates its function.

What mechanisms are in place for the reoxidation of lactate back to pyruvate?