Glycogen Debranching Enzyme Functions and Mechanism

The video tutorial explains the mechanisms of glycogen catabolism, focusing on the roles of glycogen phosphorylase and glycogen debranching enzyme. Glycogen phosphorylase removes terminal glucose units as glucose-1-phosphate, while the debranching enzyme, a hydrolase, uses water to release simple glucose. The debranching enzyme has transferase and glucosidase activities, crucial for processing branches with four glucose units. The tutorial also covers the molecular mechanism of the debranching enzyme, highlighting the differences between the two enzymes in terms of their products and processes.

What is the primary function of the glycogen debranching enzyme?

What happens to glucose units when the glycogen debranching enzyme acts on a branch with four glucose units?

What is the significance of the transferase activity of the glycogen debranching enzyme?

Describe the conditions under which glycogen phosphorylase can no longer act on a branch.

Explain the role of water in the action of the glycogen debranching enzyme.

What is the final product of the glycogen debranching enzyme's action, and how does it differ from the product of glycogen phosphorylase?

How does the mechanism of glycogen debranching enzyme differ from that of glycogen phosphorylase?