Enzyme Reactions and Lactase Extraction Techniques

This video tutorial introduces students to enzyme reactions, focusing on a method suitable for those with limited lab experience. It details the preparation of an enzyme suspension, extraction, and setting up a reaction to observe colorimetric changes. The protocol is designed to provide robust results for students to interpret.

What is the main advantage of the enzyme reaction method discussed in the video?

• It is the fastest method available.

• It is suitable for students with little lab experience.

• It uses the least amount of chemicals.

• It requires no equipment.

What is the first step in preparing the lactase enzyme suspension?

• Add ONPG solution.

• Crush a lactase tablet.

• Label a 1.5 mL tube.

• Vortex the solution.

How much of the supernatant is transferred to the tube labeled 'Lactase Extract'?

• 390 mL

• 1 mL

• 10 mL

• 500 mL

What is added to the 'Reaction A' tube after the PBS and ONPG solution?

• Water

• More PBS

• Lactase extract

• Lactase tablet

What should be observed in the reaction mixture over 5 minutes?

• pH changes

• Volume changes

• Colorimetric changes

• Temperature changes