DNA Isolation Process Techniques

This video tutorial explains the process of isolating DNA from plant tissue, specifically using onions. It covers the preparation of the onion mixture with distilled water, salt, and washing-up liquid, followed by heating and cooling to preserve DNA. The tutorial also includes blending and filtration steps to remove debris, and the addition of protease and ethanol to extract DNA. The video concludes with a summary of the steps and credits to artists.

What is the primary purpose of chopping the onion into small pieces in the DNA isolation process?

• To enhance the flavor

• To disrupt cell walls

• To increase the surface area for salt absorption

• To make it easier to blend

Why is washing-up liquid added to the solution during DNA isolation?

• To add fragrance

• To neutralize the solution

• To break down cell and nuclear membranes

• To clean the onion

What is the role of salt in the DNA isolation process?

• To dissolve the DNA

• To increase the solution's temperature

• To clump the DNA together

• To enhance the solution's color

Why is the mixture heated to 60 degrees Celsius during the DNA isolation process?

• To sterilize the solution

• To evaporate excess water

• To denature enzymes that break down DNA

• To cook the onion

What is the purpose of placing the beaker in an ice bath after heating?

• To enhance the color of the solution

• To solidify the solution

• To prevent further breakdown of DNA

• To freeze the DNA

Why is the mixture blended for only three seconds?

• To avoid destroying the DNA

• To ensure even mixing

• To create a smooth texture

• To reduce noise

What is the purpose of filtering the mixture after blending?

• To add more liquid

• To change the color

• To remove large cellular debris

• To remove DNA

What is the role of protease in the DNA isolation process?

• To change the solution's pH

• To break down proteins associated with DNA

• To enhance DNA visibility

• To dissolve the DNA

Why is ice-cold ethanol used in the final step of DNA isolation?

• To dissolve the DNA

• To make the solution colorful

• To increase the solution's temperature

• To precipitate the DNA

What is the appearance of DNA when it precipitates out of the solution?

• Solid and black

• Clear and liquid

• Powdery and yellow

• Slimy and white stringy