cDNA Synthesis and Gene Cloning

The video tutorial explains the concept of a cDNA library, its synthesis from mature mRNA, and its advantages over genomic libraries. It details the steps involved in constructing a cDNA library, highlighting the importance of cDNA in expressing eukaryotic genes in prokaryotic systems. The tutorial also compares cDNA libraries with genomic libraries, emphasizing the ease of gene expression in prokaryotic hosts when using cDNA.

What is the primary source of genes for cloning in recombinant DNA technology?

• RNA library

• Protein library

• Genomic library

• cDNA library

What is the main difference between exons and introns?

• Exons are coding, introns are non-coding

• Exons are non-coding, introns are coding

• Both are non-coding regions

• Both are coding regions

Which enzyme is responsible for synthesizing DNA from an RNA template?

• Ligase

• Reverse transcriptase

• RNA polymerase

• DNA polymerase

What is the role of ribonuclease H in cDNA synthesis?

• Joins DNA fragments

• Clips the RNA strand

• Synthesizes the first DNA strand

• Removes exons

What is the purpose of using a poly-dT primer in cDNA synthesis?

• To initiate RNA synthesis

• To bind to the poly-A tail of mRNA

• To degrade RNA

• To splice introns

Why is a cDNA library advantageous over a genomic library in prokaryotic systems?

• It is cheaper to produce

• It contains both exons and introns

• It is easier to express eukaryotic genes

• It is more stable

In a genomic library, what challenge is faced when expressing eukaryotic genes in prokaryotic hosts?

• Prokaryotic hosts cannot splice introns

• Prokaryotic hosts cannot replicate DNA

• Prokaryotic hosts cannot transcribe RNA

• Prokaryotic hosts cannot translate proteins

What is the main advantage of using cDNA for gene expression in bacteria?

• It includes introns

• It is more stable

• It is devoid of introns

• It is easier to sequence

What is the first step in constructing a cDNA library?

• Incorporation into a vector

• mRNA isolation

• Reverse transcription

• Host introduction

Which step involves introducing the recombinant plasmid into a suitable host?

• Host introduction

• Vector incorporation

• Reverse transcription

• mRNA isolation