cDNA Library Creation Concepts

This video tutorial explains the concept of cDNA libraries, highlighting their differences from genomic libraries. It details the process of creating a cDNA library, starting with mRNA isolation and reverse transcription to form cDNA. The tutorial covers the addition of restriction sites, ligation with plasmids, and transformation of bacteria. Finally, it discusses the applications of cDNA libraries in comparing protein expressions between different cells.

What is the primary difference between a genomic library and a cDNA library?

• Genomic libraries contain only expressed genes.

• cDNA libraries encompass the entire genome.

• Genomic libraries include the entire genome, while cDNA libraries focus on expressed genes.

• cDNA libraries are larger than genomic libraries.

What is the first step in creating a cDNA library?

• Ligation of cDNA with plasmids

• Isolating messenger RNA

• Transforming bacteria

• Adding restriction sites

Why is messenger RNA isolated in the process of creating a cDNA library?

• It contains the entire genome.

• It is the RNA that is expressed in the cell.

• It is more stable than DNA.

• It is easier to work with than DNA.

What technique is used to create DNA from RNA in the cDNA library process?

• Gel electrophoresis

• DNA sequencing

• Reverse transcription

• Polymerase chain reaction

What is the purpose of adding restriction sites to cDNA?

• To degrade the cDNA

• To sequence the cDNA

• To ensure cDNA remains intact

• To transform the cDNA

Which enzyme is used to ligate cDNA with plasmids?

• DNA polymerase

• RNA ligase

• DNA ligase

• Reverse transcriptase

What is the role of DNA ligase in the cDNA library creation process?

• It transcribes RNA into DNA.

• It cuts the DNA at specific sites.

• It isolates mRNA from the cell.

• It combines cut plasmid and cDNA to form a complete plasmid.

What is the final step in creating a cDNA library?

• Reverse transcription

• Adding restriction sites

• Isolating mRNA

• Transforming bacteria

How can cDNA libraries be useful in research?

• They help in sequencing the entire genome.

• They allow comparison of protein expressions between different cells.

• They are used to degrade unwanted DNA.

• They are used to clone entire organisms.

What forms the cDNA library after transforming bacteria?

• Only plasmids without cDNA

• A single type of cDNA

• Only genomic DNA

• A combination of cells with different types of cDNA