cDNA Library Creation Concepts

Genomic libraries contain only expressed genes.

cDNA libraries encompass the entire genome.

Genomic libraries include the entire genome, while cDNA libraries focus on expressed genes.

cDNA libraries are larger than genomic libraries.

Ligation of cDNA with plasmids

Isolating messenger RNA

Transforming bacteria

Adding restriction sites

It contains the entire genome.

It is the RNA that is expressed in the cell.

It is more stable than DNA.

It is easier to work with than DNA.

Gel electrophoresis

DNA sequencing

Reverse transcription

Polymerase chain reaction

To degrade the cDNA

To sequence the cDNA

To ensure cDNA remains intact

To transform the cDNA

DNA polymerase

RNA ligase

DNA ligase

Reverse transcriptase

It transcribes RNA into DNA.

It cuts the DNA at specific sites.

It isolates mRNA from the cell.

It combines cut plasmid and cDNA to form a complete plasmid.

Reverse transcription

Adding restriction sites

Isolating mRNA

Transforming bacteria

They help in sequencing the entire genome.

They allow comparison of protein expressions between different cells.

They are used to degrade unwanted DNA.

They are used to clone entire organisms.

Only plasmids without cDNA

A single type of cDNA

Only genomic DNA

A combination of cells with different types of cDNA