Microscope Limitations and Functions

The video tutorial covers methods of studying cells, including the use of microscopes and ultracentrifugation. It provides tips for improving scientific drawings and explains the process of cell fractionation. The importance of buffer solutions in maintaining cell integrity is discussed, along with a comparison of different types of microscopes, such as transmission and scanning electron microscopes. The tutorial concludes with an overview of the principles and limitations of electron microscopy.

What is one of the key tools mentioned for studying cells?

• Thermometer

• Eyepiece graticule

• Stethoscope

• Barometer

What is the primary purpose of cell fractionation?

• To increase cell size

• To separate organelles

• To decrease cell size

• To color the cells

Why is an ice-cold buffer solution used in cell fractionation?

• To change the color of organelles

• To speed up reactions

• To increase enzyme activity

• To reduce enzyme activity

What is the purpose of using an isotonic solution during cell fractionation?

• To prevent organelle damage

• To speed up organelle movement

• To change organelle color

• To increase organelle size

What is the first step in calculating magnification?

• Convert units to micrometers

• Measure with a ruler

• Multiply by the given magnification

• Divide by the given magnification

Which type of microscope uses electrons instead of light?

• Transmission electron microscope

• Simple microscope

• Optical microscope

• Compound microscope

What is a limitation of the transmission electron microscope?

• Simple preparation

• Requires thin specimens

• Can view living material

• Shows color images

Which microscope can provide a 3D image?

• Optical microscope

• Scanning electron microscope

• Simple microscope

• Transmission electron microscope

What is a key difference between TEM and optical microscopes?

• TEM can view live specimens, optical cannot

• TEM shows color images, optical does not

• TEM requires complex preparation, optical does not

• TEM uses light, optical uses electrons

Why is it important to know the limitations of different microscopes?

• To ensure all microscopes are used equally

• To choose the right microscope for specific research needs

• To increase the cost of research

• To avoid using microscopes altogether