Gene Selection and Editing Procedures

This video tutorial guides users through the process of designing and ordering reagents for gene editing using the Invitrogen TrueDesign Genome Editor. It covers logging into the Thermo Fisher Connect platform, selecting experimental options, choosing genes, and editing sequences. The tutorial also explains how to insert and delete DNA sequences, select guide RNAs and TALEN targets, and finalize the order of necessary reagents.

What is the first step after logging into the Thermo Fisher Connect platform?

• Select the gene of interest

• Choose the type of experiment

• Access the True Design Genome Editor

• Order the necessary reagents

Which option allows you to insert up to 30 base pairs of new DNA?

• Insertion

• Duplication

• Deletion

• Substitution

How do you identify your gene of interest in the platform?

• By uploading a DNA sequence

• By entering the gene symbol or Entrez ID

• By selecting the species

• By choosing the experiment type

What can you do if you are unsure about which transcript to select?

• Skip the selection

• Guess based on the gene name

• Use the transcript ID hyperlink to visit the NCBI website

• Consult a colleague

What color highlights the inserted bases in the sequence editor?

• Yellow

• Blue

• Green

• Red

What is the purpose of the blue undo arrow in the edit list?

• To save the changes

• To highlight the changes

• To redo the changes

• To undo the changes

What does the software search for during the design step?

• Available PAM sites and guide RNA designs

• Gene symbols and Entrez IDs

• Host organisms and species

• DNA sequences and oligos

What information is displayed in the CRISPR table?

• Gene symbols and Entrez IDs

• CRISPR guide RNA design, PAM site, and score

• Host organism and species

• DNA sequences and oligos

What additional items can be added to the experiment?

• DNA sequences and oligos

• Sequencing primers and experimental controls

• Gene symbols and Entrez IDs

• Host organisms and species

What can you do after completing your product selections?

• Add to cart for ordering

• Log out of the platform

• Delete the experiment

• Restart the process