Plasmid Isolation Techniques and Principles

To degrade RNA present in the mixture

To enhance the growth of bacterial culture

To stabilize the plasmid DNA

To increase the viscosity of the solution

To ensure even spinning and prevent damage

To reduce noise during operation

To prevent the centrifuge from overheating

To increase the speed of centrifugation

Room temperature

4°C

37°C

100°C

To precipitate proteins

To neutralize the pH of the solution

To dissolve the cell wall and release plasmid DNA

To enhance the color of the solution

Ten times

Five to six times

Three times

Once

To heat the solution

To mix the solution thoroughly

To cool the solution

To separate the components

To dissolve the plasmid DNA

To precipitate the plasmid DNA

To increase the temperature of the solution

To act as a buffer

To change the color of the pellet

To increase the concentration of plasmid DNA

To enhance the stability of the pellet

To remove any remaining ethanol

Observing under a microscope

Using a spectrophotometer

Performing agarose gel electrophoresis

Measuring the pH of the solution

To stabilize the gel matrix

To identify the size of the plasmid

To enhance the visibility of the gel

To increase the speed of electrophoresis