Microscope Functionality and Usage

The video tutorial provides an overview of microscope parts and their functions, focusing on magnification, field of view, and depth of field. It explains how to calculate total magnification by multiplying ocular and objective lens powers. The tutorial also covers practical lab instructions for using a microscope, including focusing techniques and understanding image inversion. Key concepts such as resolution, field of view, and depth of field are discussed, with comparisons to camera zooming to aid understanding.

What is the primary purpose of using different magnifications in a microscope?

• To adjust the size of the microscope

• To see smaller objects more clearly

• To increase the weight of the microscope

• To change the color of the specimen

How do you calculate the total magnification of a microscope?

• Subtract the power of the ocular lens from the objective lens

• Multiply the powers of the ocular and objective lenses

• Divide the power of the objective lens by the ocular lens

• Add the powers of the ocular and objective lenses

What happens to the field of view as magnification increases?

• It remains the same

• It decreases

• It becomes square

• It increases

Why is the field of view important when using a microscope?

• It determines the color of the specimen

• It increases the weight of the microscope

• It helps estimate the size of objects within view

• It changes the shape of the specimen

What is the depth of field in microscopy?

• The weight of the microscope

• The thickness of the specimen in focus at one time

• The total magnification of the microscope

• The amount of light passing through the specimen

How does the depth of field change as magnification increases?

• It remains the same

• It decreases

• It increases

• It becomes infinite

What is the first step in focusing a microscope on a slide?

• Start with the highest magnification

• Adjust the light source

• Move the stage to the lowest position

• Start with the scanning power at 4x

What does it mean when an image is inverted under a microscope?

• The image is upside down and backwards

• The image is in black and white

• The image is in color

• The image is larger than the specimen

Why should you avoid using the coarse focus knob at higher magnifications?

• It can break the slide

• It can make the image too bright

• It can cause you to lose focus on the object

• It can change the color of the specimen

What adjustment might be necessary as you increase magnification to maintain image clarity?

• Rotate the microscope

• Change the color of the light

• Adjust the condenser diaphragm

• Increase the weight of the microscope