Gel Electrophoresis Techniques

This video tutorial guides you through the process of casting an agarose gel. It begins with preparing the gel tray by taping its ends to prevent leaks. Next, it explains how to place the comb in the tray for DNA sample wells. The video then covers pouring molten agarose into the tray and ensuring it cools properly. After the gel sets, the comb is removed to reveal well-formed wells. Finally, instructions are provided for storing the gel if not used immediately.

What is the purpose of using tape on the gel tray?

• To change its color

• To make it heavier

• To prevent leaks

• To decorate the tray

Where should the comb be placed if you plan to run DNA samples?

• Under the gel tray

• In the middle of the gel tray

• At one end of the gel tray

• On top of the gel tray

What temperature should the agarose be cooled to before pouring?

• 75° C

• 65° C

• 55° C

• 45° C

How close should the agarose be poured to the top of the comb's teeth?

• 1 to 2 mm

• 6 to 8 mm

• 2 to 4 mm

• 4 to 6 mm

What should you do if the gel is to be used immediately after setting?

• Store it at 4° C

• Remove the tape and continue with the activity

• Leave it in the tray

• Cover it with a plastic bag

What is the correct way to remove the comb from the gel?

• Pull it gently straight up

• Push it sideways

• Twist it out

• Lift it at an angle

What happens if the comb is removed before the gel is set?

• The wells will expand

• The gel will melt

• The wells will collapse

• The gel will harden

How should the gel be stored if not used immediately?

• At room temperature

• In direct sunlight

• In a small plastic bag at 4° C

• In a freezer