Electrophoresis Experiment Procedures

This instructional video guides viewers through performing electrophoresis in five steps. It begins with an introduction to electrophoresis and preparation of the agarose gel tray. The video then details each step: setting up the gel, loading dye samples, ensuring safety and orientation, connecting power to perform electrophoresis, and finally visualizing the results. The process is demonstrated with clear instructions and visual aids, making it easy to follow and understand.

What is the first step in setting up the electrophoresis experiment?

• Visualize the results.

• Connect the leads to the power source.

• Place the gel tray into the electrophoresis chamber.

• Load dye samples into the wells.

How much dye sample should be loaded into each well?

• 40 to 45 µL

• 35 to 38 µL

• 30 to 32 µL

• 20 to 25 µL

In which direction do the dye samples migrate during electrophoresis?

• Towards the negative electrode

• In random directions

• Towards the positive electrode

• They do not migrate

What should be done after electrophoresis is complete?

• Stain the gel

• Remove the gel and visualize the results

• Load more dye samples

• Increase the voltage

Is staining necessary to visualize the results after electrophoresis?

• Only if the gel is not clear

• Only for certain samples

• No, it is not necessary

• Yes, always