Gel Electrophoresis Procedures

The video tutorial provides a comprehensive guide on setting up and using the Lonza system for gel electrophoresis. It covers the necessary components, equipment setup, gel preparation, sample loading, running the gel, and capturing results. The tutorial emphasizes proper connectivity, safety, and accuracy in each step to ensure successful visualization of DNA samples.

Which of the following is NOT a component required for the Lonza system setup?

• Camera Hood

• Power Supply

• Microscope

• Laptop

What should you do before running the gel with the cords?

• Plug them into the wall outlet

• Connect them to the laptop

• Label them for connectivity

• Plug them into the power supply immediately

What is necessary for the laptop to power on?

• A USB connection to the camera hood

• A wireless connection

• Being plugged into a wall power outlet

• A fully charged battery

What should be done to the wells before loading the gel with samples?

• Wash them with water

• Add a DNA ladder

• Fill them with gel

• Heat them

What color should the solutions be to indicate they are ready for visualization?

• Blue or green

• Red or purple

• Yellow or orange

• Black or white

Where should the DNA ladder be loaded in the gel?

• In the last well

• In the first well

• In the middle well

• In any well

What is the correct way to expel a sample into the well?

• Quickly to avoid bubbles

• Slowly to avoid bubbles

• With the tip hovering above the well

• With the tip jammed to the bottom

At what voltage should the power supply be set for running the gel?

• 275 volts

• 200 volts

• 300 volts

• 150 volts

What should you do to see the DNA bands migrating through the gel in real time?

• Use a microscope

• Press the light button on the gel dock

• Increase the voltage

• Add more dye

What is the final step after capturing an image of the gel?

• Transfer the image to your own storage device

• Unplug the camera hood

• Turn off the blue light

• Re-run the gel electrophoresis