Dried Blood Spot Procedures and Safety

This video tutorial explains the process of collecting and storing dried blood spots for genetic analysis. It covers the materials required, safety precautions, and detailed steps for labeling, applying, drying, and storing the blood spots. The procedure involves using FTA cards and specific volumes of blood, with emphasis on proper handling and storage to prevent contamination and moisture issues.

What is the primary purpose of collecting dried blood spots?

• For blood type determination

• To analyze blood sugar levels

• For genetic analysis

• To measure cholesterol

Which of the following is NOT required for the dried blood spot procedure?

• Watman FTA classic cards

• Bleach solution

• Refrigerator

• 200 microl pipet tips

What is a key safety precaution when handling blood samples?

• Storing samples in sunlight

• Wearing protective eyewear

• Using a microwave

• Applying heat to dry samples

How much blood should be applied to each circle on the FTA card from a venous blood draw?

• 200 microl

• 150 microl

• 50 microl

• 100 microl

What is the minimum total volume of blood required when using a finger stick?

• 200 microl

• 150 microl

• 100 microl

• 250 microl

What is the correct method to apply blood to the FTA card?

• Start from the edge and move inward

• Apply in a zigzag pattern

• Apply randomly

• Begin at the center and move out in a spiral

How long should the FTA card be suspended at room temperature to dry?

• 8 hours

• 4 hours

• 6 hours

• 2 hours

Why should FTA cards not be refrigerated or frozen?

• It changes the color of the blood spots

• It makes the card too brittle

• It can lead to moisture condensation

• It causes the card to shrink

What should be done after drying the FTA card?

• Apply heat to ensure dryness

• Place in direct sunlight

• Store in a refrigerator

• Seal in a zip-lock bag with a desiccant

What is the purpose of using a desiccant pack with the FTA card?

• To absorb moisture

• To prevent mold growth

• To keep the card warm

• To add moisture