Interpreting an Affinity Chromatogram

The video tutorial explains the concept of chromatograms and their interpretation, focusing on affinity chromatography. It covers the principles of affinity chromatography, using examples with enzymes like hexokinase and lactate dehydrogenase. The tutorial details how proteins with specific affinities are separated using columns with analogs of substrates or coenzymes. It also discusses the analysis of chromatograms, identifying peaks, and the process of using wash and elution buffers to purify proteins. The video concludes with a discussion on the importance of affinity chromatography in protein purification.

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