Microscope Types and Their Uses

What is the maximum magnification of a simple microscope like a magnifying glass?

• 100x

• 50x

• 10x

• 5x

Which two lenses are part of a compound microscope?

• Upper and Lower

• Primary and Secondary

• Ocular and Objective

• Convex and Concave

What is the main advantage of using a dark field microscope?

• Low cost

• High magnification

• Viewing transparent specimens

• Viewing stained specimens

What is the purpose of the condenser annulus in a phase contrast microscope?

• To focus the light

• To create a hollow cone of light

• To stain the specimen

• To magnify the specimen

What type of images does a differential interference contrast microscope produce?

• 2D images

• Black and white images

• 3D-like images

• Fluorescent images

What is a key requirement for using a fluorescence microscope?

• Fluorescent tagging of specimens

• High power source

• Large specimens

• Low magnification

What is the primary difference between a confocal and a normal fluorescence microscope?

• Use of a pinhole

• Larger size

• Higher magnification

• Lower cost

What is the illumination source in a scanning electron microscope?

• Electron beam

• X-ray beam

• Laser beam

• Light beam

What is a major limitation of a transmission electron microscope?

• High cost

• Low magnification

• Requires thick samples

• Cannot view live specimens

What is the benefit of a scanning transmission electron microscope (STEM)?

• Simple operation

• Combines features of SEM and TEM

• Requires no sample preparation

• Low cost