10 Common mistakes when using a microscope

Why is it recommended to start microscopy with a low-power objective?

• To prevent damage to the high-power objectives.

• To achieve a higher magnification immediately.

• To easily focus and center the specimen due to a larger depth of field.

• To reduce the amount of light entering the microscope.

What is a common issue when the specimen is not centered on the microscope slide?

• The image appears too bright.

• The specimen cannot be found or is partially visible, often showing the slide label instead.

• The microscope's light source will flicker.

• The objective lens will become scratched.

What is the effect of starting microscopy with the condenser fully open?

• It increases the depth of field, making focusing easier.

• It provides maximum contrast for detailed viewing.

• It reduces contrast and decreases the depth of field, making focusing difficult.

• It protects the objective lenses from damage.

What is the primary risk of using immersion oil with a "dry objective" (non-oil objective)?

• It will make the image appear blurry.

• It can permanently damage the objective lens.

• It will cause the specimen to dry out quickly.

• It will increase the magnification beyond its intended limit.

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What is a potential consequence of using mounting medium instead of immersion oil with a microscope objective?

• The objective may become damaged.

• The image clarity will significantly improve.

• The slide will be easier to clean after use.

• The lifespan of the objective will be extended.

Why is it advised to avoid using the coarse focus knob with high-power objectives?

• It can cause the objective to crash into and damage the specimen slide.

• It makes the specimen appear too bright, hindering observation.

• It is exclusively designed for low-power magnification.

• It can lead to the microscope overheating.

If focus is lost while observing a specimen, what is the recommended method to regain it efficiently?

• Rotate to a low-power objective and use the coarse focus knob.

• Rapidly spin the fine focus knob in both directions.

• Adjust the light intensity to make the specimen clearer.

• Remove and clean the objective lens.

What is the primary function of the condenser diaphragm on a microscope?

• To control the contrast and depth of field.

• To adjust the overall brightness of the light source.

• To fine-tune the focus of the image.

• To change the magnification level.