WorksheetsChapter 9 Biotechnology
Total questions: 20
Worksheet time: 11mins
Name
Class
Date
1.
A restriction enzyme cuts a segment of DNA into 8 pieces. How many restriction sites are there?
a)
6
b)
7
c)
8
d)
9
2.
A type of restriction map, called a DNA fingerprint, compares the ____ of the ____ of DNA.
a)
base pairs; noncoding regions
b)
sizes; coding regions
c)
lengths; coding regions
d)
sizes, noncoding regions
3.
Why are primers necessary for PCR?
a)
They help separate the double helix
b)
They are a starting point for DNA polymerase
c)
They help anneal the newly formed strands
d)
They bring in new nucleotides
4.
In gel electrophoresis, the largest DNA fragment will appear
a)
closest to the starting wells
b)
farthest from the starting wells
c)
three quarters away from the starting wells
d)
it depends on how many fragments there are
5.
What is necessary for the polymerase chain reaction to occur?
a)
DNA polymerase + primers
b)
RNA polymerase + DNA sequence
c)
DNA polymerase + mRNA sequence
d)
RNA polymerase + free floating nucleotides
6.
We can figure out which genes are expressed at the same time by observing
a)
a DNA fingerprint
b)
a restriction map
c)
a microarray
d)
a piece of recombinant DNA
7.
Genetic engineering involves ___ while gene therapy involves ___.
a)
a bacterial plasmid, restriction enzymes
b)
a bacterial plasmid; a virus
c)
restriction enzymes; a virus
d)
a virus; a bacterial plasmid
8.
During gel electrophoresis, DNA moves from the ___ end to the ___ end.
a)
positive, negative
b)
negative, positive
9.
Compared to sticky ends, which are exposed bases, blunt ends___
a)
are made of repeating bases
b)
are paired bases
c)
are restricted bases
d)
are made of non complementary bases
10.
To genetically engineer a transgenic organism, the foreign DNA & organism's DNA must contain
a)
repeating nucleotides
b)
different nitrogen bases
c)
the same nitrogenous bases
d)
noncoding regions of DNA
11.
By looking at a restriction map, we can tell the size, but NOT the DNA sequences themselves.
a)
True
b)
False
12.
Genomics studies ___ and proteomics studies ___.
a)
genotypes; proteins
b)
proteins; genes
c)
genes; proteins
d)
genes; phenotypes
13.
Sequencing ALL of our DNA & identifying ALL of the genes in our genome are the 2 goals of the
a)
HapMap Project
b)
DNA Fingerprint Project
c)
Proteome Project
d)
Human Genome Project
14.
Restriction enzymes are naturally occurring in
a)
yeast
b)
humans
c)
viruses
d)
bacteria
15.
DNA fingerprints help identify people at the ___ level
a)
molecular
b)
cellular
c)
atomic
d)
macroscopic
16.
We can treat some genetic disorders by replacing ___ or ___ genes, which is called gene therapy
a)
recessive, duplicated
b)
defective, missing
c)
missing, linked
d)
defective, duplicated
17.
Knocking out a gene is equivalent to ___, which helps us determine gene function.
a)
rearranging the base pairs
b)
separating the complementary DNA
c)
turning it off
d)
turning it on
18.
One risk (consequence) of genetically engineering plants could be
a)
disease resistance
b)
decreased biodiversity
c)
resistance to frost
d)
produce vitamins
19.
You want to have a child, but you are unsure of the likelihood of passing on the trait for cystic fibrosis. What can you do to figure out the chances of passing this trait on?
a)
gene therapy
b)
PCR
c)
genetic screening
d)
genome sequencing
20.
What is the probability that two people have the same number of noncoding repeats in both location A & location B, as shown on a DNA fingerprint, if the chance that they both have 4 repeat sequences at location A is 1/10 and the chance that they both have six repeats at location B is 1/2,000?
a)
1/200,000
b)
1/2,010
c)
1/2,000
d)
1/20,000
100 %
