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WorksheetsMolecular Microbiology
Total questions: 20
Worksheet time: 10mins
Name
Class
Date
1.
What is the backbone of DNA made of?
a)
Glucose and Nitrogen
b)
Deoxyribose and Nitrogen
c)
Deoxyribose and Phosphate
d)
Deoxyribose and Sulfate
2.
What kind of bonds hold the DNA molecule together?
a)
Phosphate bonds and Hydrogen bonds
b)
Dipolar bonds and Hydrogen bonds
c)
Hydrogen bonds an Sulfate bonds
d)
Covalent bonds and Hydrogen bonds
3.
Which bases pair together in the DNA molecule?
a)
A-G and C-T
b)
A-T and C-G
c)
A-C and G-T
d)
A-U and G-C
4.
Which best describes an Okazaki fragment?
a)
A piece of single strand DNA that gets replicated into double strand DNA
b)
A small piece of RNA attached to the DNA strand to start replication.
c)
Complementary pieces of DNA that match to the lagging strand
d)
A DNA primer that matches the target DNA
5.
Which enzyme seals the fragments of DNA into a continuous strand?
a)
DNA Ligase
b)
DNA Polymerase
c)
Helicase
d)
Exonuclease
6.
Which of the following excises introns from the mRNA strand?
a)
tRNA
b)
Exons
c)
Spliceosome
d)
RNA Polymerase
7.
Which enzyme is responsible for elongating the mRNA strand?
a)
DNA polymerase
b)
RNA polymerase
c)
Promoter region
d)
Ribosome
8.
Which enzyme begins DNA replication with a small strand of RNA?
a)
Helicase
b)
Primase
c)
DNA Ligase
d)
DNA Polymerase
9.
The Gene Expert cartridge allows for which of the following?
a)
All PCR reagents are contained within one cartridge
b)
Mutiplex PCR for multiple targets
c)
PCR amplification of RNA viruses
d)
Nested PCR
10.
Which enzyme adds base pairs to elongate single stranded DNA?
a)
Exonuclease
b)
DNA Ligase
c)
Primase
d)
DNA polymerase
11.
What is RFLP used for?
a)
Detecting PCR products
b)
Amplify nucleic acids
c)
Used for Eastern blot
d)
DNA fingerprinting
12.
Which step in PCR is responsible for hybridization?
a)
Annealing
b)
Initiation
c)
Denaturation
d)
Extension
13.
Why is Taq used in PCR?
a)
It is a specific DNA Primer
b)
It is a heat stable polymerase
c)
It is specific to the target probe
d)
It does not need a cofactor
14.
What is RT-PCR used for?
a)
To detect PCR products as they are produced.
b)
To quantify PCR products
c)
To detect a viral load
d)
To amplify RNA viruses
15.
Which of the following techniques allows for mutiplexing?
a)
Branched DNA
b)
Nested PCR
c)
Real time PCR
d)
Reverse Transcriptase PCR
16.
Which of the following results in an amino acid substitution?
a)
Deletion
b)
Insertion
c)
Nonsense
d)
Missense
17.
What is the major drawback of the BioFire Film Array?
a)
It can only detect one PCR target
b)
It cannot detect slowgrowing organisms
c)
It can only do one sample per hour
d)
It cannot detect resistance genes
18.
What advantage does the MALDI-TOF provide?
a)
It takes minutes instead of days for identification of pathogens
b)
It can detect multiple targets
c)
It is inexpensive
d)
It can detect antibiotic resistance
19.
What testing principle is utilized by the MALDI-TOF?
a)
PCR
b)
RT-PCR
c)
Multiplex PCR
d)
Mass Spectrometry
20.
In gel electrophoresis, what separates the DNA fragments?
a)
They are separated by an electrical field
b)
They are separated by magnetic fields
c)
They are separated by binding to the gel
d)
They are separated by the electrical charge of the gel
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