WorksheetsAseptic Technique
Total questions: 14
Worksheet time: 12mins
Name
Class
Date
1.
The purpose of streaking a plate is to obtain
a)
large quantities of bacteria
b)
bacteria in pure culture
2.
When streaking a plate, primarily
a)
flex and extend the fingers
b)
move from the wrist
c)
move your whole arm
3.
Plates should be incubated
a)
agar side up
b)
agar side down
c)
it doesn't matter
4.
When streaking a plate, you should go back over the last area
a)
never
b)
1-2 times
c)
3-4 times
d)
8-10 times
5.
When streaking a plate, the lid should be
a)
placed on the bench top, top side up
b)
placed on the bench top, inside surface up
c)
should be held in the same hand as the plate
d)
should be held in the opposite hand from the plate
6.
When streaking a plate, the loop should be flamed
a)
At the beginning and end
b)
At the beginning and end and between the first 2 zones
c)
At the beginning and end and between the second and third zone
d)
At the beginning and end and between each zone
7.
George streaks a plate of medium from a broth culture. After incubation, he sees confluent growth in all sectors. Which of the following errors could NOT explain this result?
a)
He forgot to pick a colony from the plate
b)
He forgot to flame the loop between sectors
c)
He picked additional culture from the plate after the first sector
d)
He crossed over the previous streak too many times at the beginning of a new sector.
8.
Which finger should you use to remove the lid from a test tube when you are using an inoculating loop to make a transfer?
a)
little finger of the dominant hand (hand holding the loop)
b)
little finger of the non-dominant hand (hand holding the sample)
c)
forefinger of the dominant hand (hand holding the loop)
d)
thumb and forefinger of the non-dominant hand (hand holding the sample)
9.
The bacteriology loop should be sterilized in what part of the Bunsen burner flame?
a)
in the bright blue cone
b)
just above the bright blue cone
c)
above the flame
d)
at the base of the flame
10.
The loop should be held
a)
straight up and down
b)
at a very slight angle
c)
at a fairly steep angle
d)
parallel to the work bench
11.
When transferring liquid using a pipette, the tip of the pipette should
a)
be in the center of the test tube
b)
should touch the side of the test tube
c)
never touch the side of the test tube
d)
remain in the air above the tube and never enter the tube.
12.
When transferring liquid aseptically from one tube to another, you should
a)
remove the cap of the tube with the little finger of the hand holding the pipette
b)
remove the cap of the tube with the little finger of the hand holding the tube
c)
place the cap on the bench top with the inside up
d)
place the cap on the bench top with the inside down
13.
What is the first thing you should do?
a)
put on gloves
b)
wash your hands
c)
sterilize your workspace
14.
What is the last thing you should do?
a)
take off your gloves
b)
wash your hands
c)
sterilize your workspace
100 %
