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Nitrogen fixation - N and S assimilation & anaplerotic reaction

Total questions: 17

Worksheet time: 17mins

Name
Class
Date
1.

Nitrogen fixation is an

a)

anaerobic process

b)

aerobic process

c)

microaerophilic process

d)

aeroleterant process

2.

N2 fixation that takes place under high temperature and pressure, in the presence of Fe or Ru as catalyst is

a)

Biological N fixation

b)

Industrial N fixation

c)

both

d)

atmospheric N fixation

3.

The nitrogen fixation organisms are….A….. and are commonly called as….B……

a)

A- Prokaryotes or eukaryotes and B - diazotrophs

b)

A- Prokaryotes and B - piezotrophs

c)

A- Prokaryotes and B - diazotrophs

d)

A-eukaryotes and B-piezotrophs

4.

An example for associative symbiotic nitrogen fixer

a)

Rhizobium

b)

Azotobacter

c)

Clostridium

d)

Azospirillum

5.

The amount of biological nitrogen fixed annually is

a)

50-100 terra g N / year

b)

100-140 terra g N / year

c)

100-200 terra g N / year

d)

<50 terra g N / year

6.

Which of the following process could mediate atmospheric nitrogen fixation

a)

cosmic radiation

b)

meteorite trails

c)

lightning

d)

all the above

7.

Nif genes are those that codes for

a)

nitrogenase enzyme

b)

nitrate reductase

c)

nitric oxide reductase

d)

Urease

8.

identify the CORRECT statements related to nitrogenase enzyme

(i) highly substrate specific

(ii) Reqs Fe & Mo for its activity

(iii) commonly it functions under low and high temperatures

(iv) reqs. 14-16 ATP for their activity

a)

both (i) and (ii)

b)

(i) only

c)

(ii) & (iv)

d)

(iii) & (iv)

9.

A cation that is much essential for the activity of the nitrogenase enzyme is

a)

Ca2+

b)

Mg2+

c)

Na

d)

K

10.

An example for symbiotic nitrogen fixer

a)

Rhizobium

b)

Azotobacter

c)

Clostridium

d)

Azospirillum

11.

Enzyme(s) commonly invovled in N assimilation is

a)

glutamate dehydrogenase

b)

glutamine synthase

c)

glutamate synthase

d)

All the three

e)

nitrogenase

12.

An example for anaerobic nitrogen fixer

a)

Rhizobium

b)

Azotobacter

c)

Clostridium

d)

Azospirillum

13.

Enzyme diamide and enzyme hydrazine refers to

a)

enzyme-substrate intermediate forms resulted during N2 fixation

b)

forms of enzymes involved in N2 fixation

c)

Alternative nitrogenase forms

d)

changes in shape of enzyme after bonding of N2

14.

Acetylene, cyanide and carbon monoxide are

a)

subrates for nitrogenase

b)

poison the functioning of nitrogenase

c)

coenzyme or co-factors of the nitrogenase

d)

can be substrates or poisons depending upon the conditions

15.

Identify the CORRECT statements related to nitrogenase enzyme

(i) it is a primitive enzyme like rhodonase

(ii) it can also use proton and electron as substrate to produce H2

(iii) it is coded by nif genes

(iv) expression of nif genes in the plant by rDNA technology is a currently available technology

a)

All the statements are correct

b)

(i), (ii) & (iii)

c)

(i) and (ii)

d)

(iv) alone

16.

The main protein of the nitrogenase are coded by the genes

a)

nif AL

b)

nif HDK

c)

nif F

d)

nif XNE

17.

Nitrogenase is subjected to regulation controls by

a)

O2

b)

NH3

c)

NO3

d)

Nif L gene products

e)

all the above