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Acid Fast Staining

Total questions: 15

Worksheet time: 5mins

Name
Class
Date
1.

Exhibits acid fast characteristic

a)

mycolic acid

b)

acid alcohol

c)

fast motility

d)

thin cell wall

2.

Positive result for most AFS stains

a)

blue

b)

red

c)

purple

d)

pink

3.

Kinyoun's cold stain is performed without ______.

a)

staining

b)

decolorizing

c)

steaming

d)

drying

4.

The modification in Cooper's modified procedure

a)

Cooper Brilliant Green

b)

Copper Brilliant Green

c)

Cooper Balsamic Green

d)

Cooper Malachite Green

5.

Uses rosolic acid in alcohol

a)

Gabbett's modification

b)

Ziehl Neelsen AFS

c)

Pappenheim's method

d)

Muller-Chermock CFT

6.

Baumgarten's method differentiates_________ from M. tuberculosis

a)

M. leprae

b)

M. smegmatis

c)

M. laticola

d)

M. inem

7.

Stains the M. leprae red

a)

Dilute alcholic fuchsin

b)

Dilute acid alcohol

c)

Dilute acid fuchsin

d)

Rosolic acid in alcohol

8.

Decolorizes M. laticola and M. smegmatis

a)

Rosolic acid in alcohol

b)

Dilute alcoholic fuchsin

c)

Acid alcohol

d)

Methylene blue

9.

M. tuberculosis in Baumgarten's method

a)

Blue

b)

Red

c)

Purple

d)

Pink

10.

Objective used in Truant Fluorochrome Stain

a)

Scanner

b)

Low Power Objective

c)

High Power Objective

d)

Oil Immersion Objective

11.

Counterstain for fluorochrome stain

a)

Potassium permanganate

b)

Acridine orange

c)

Methylene blue

d)

Malachite green

12.

Contrasts fluorescence stain background

a)

Potassium permanganate

b)

Acridine orange

c)

Methylene blue

d)

Malachite green

13.

Exhibits fluorescence of acid fast microorganism

a)

Auramine orange

b)

Potassium permanganate

c)

Acridine orange

d)

Acid Alcohol

14.

Non-acid fast color upon decolorization

a)

Red

b)

Green

c)

None

d)

Blue

15.

Non-acid fast microorganism

a)

Legionella

b)

Cryptosporidium

c)

Corynebacterium

d)

Rhodococcus