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Section 1

Total questions: 159

Worksheet time: 1hrs 20mins

Name
Class
Date
1.

The principle of any biochemical test depends on........

a)

Enzyme

b)

Substrate

c)

Reagent

d)

All the above

e)

None of the above

2.

Catalase test used to detect bacterial ability to produce.....

a)

Catalase

b)

urease

c)

Oxidase

d)

Coagulase

e)

None of the above

3.

Catalase test used to detect bacterial ability to split.....

a)

H2O

b)

O2

c)

O3

d)

H2O2

4.

Catalase test reagent is.....

a)

H2O2

b)

Hydrogen peroxide

c)

All of the above

d)

None of the above

5.

Which of the following is catalase positive.....

a)

Staphylococcus aureus

b)

Staphylococcus epidermidis

c)

Staphylococcus citrus

d)

Staphylococcus albus

e)

Staphylococcus saprophyticus

6.

Which of the following is Catalase negative....

a)

Streptococcus pyogens

b)

Staphylococcus intermedius

c)

Streptococcus agalactiae

d)

Staphylococcus hyacius

e)

Streptococcus equi subspp equi

7.

Catalase test able to differentiate between........

a)

Staphylococcus species and Enterobacteriaceae family

b)

Staphylococcus species and Baillaceae family

c)

Staphylococcus species and streptococcus species

8.

Positive catalase appear as.........

a)

clot formation

b)

liquefaction

c)

effervescence

9.

Oxidase test detect bacterial ability to produce........

a)

Catalase

b)

oxidase

c)

Coagulase

d)

urease

10.

The oxidase reagent is...........

a)

sulfanilic acid

b)

alpha naphthylamine

c)

Kovac's reagent

d)

1% para-aminodimethyl aniline oxalate

11.

Positive oxidase appear as.......

a)

blue color

b)

Mint green color

c)

orange color

d)

pink color

e)

purple color

12.

Oxidase test used to differentiate between......

a)

Enterobacteriaceae and non enterobacteriaceae

b)

Pseudomonas genus and Enterobacteriaceae

c)

All of the above

d)

Non of the above

13.

Coagulase test detect bacterial ability to produce......

a)

urease

b)

oxidase

c)

coagulase

d)

catalase

14.

Coagulase positive appears as.....

a)

clot formation

b)

liquefaction

c)

effervescence

d)

red ring

e)

purple color

15.

which of the following is Coagulase positive

a)

S.aureus

b)

S.epidermidis

c)

S.saprophyticus

d)

S.hyicus

e)

S.intermedius

16.

which of the following is tube Coagulase negative

a)

S.aureus

b)

S.epidermidis

c)

S.saprophyticus

d)

S.hyicus

e)

S.intermedius

17.

Which of the following can be used as substrate in tube coagulase test

a)

rabbit plasma

b)

human plasma

c)

mice plasma

d)

fowl plasma

18.

Which of the following can be used as substrate in slide coagulase test

a)

rabbit plasma

b)

human plasma

c)

mice plasma

d)

fowl plasma

19.

The incubation period of Coagulase test is.......(in case of +ve result)

a)

24 hr

b)

4 hr

c)

4 min

d)

overnight

20.

The incubation period of Coagulase test is.......(in case of -ve result)

a)

24 hr

b)

4 hr

c)

4 min

d)

overnight

21.

Sugar(CHO) fermentation test used to detect the ability of bacteria to...........

a)

ferment certain type of sugar and production of acid or acid+gas

b)

ferment of mannitol and production of acid or acid+gas

c)

ferment lactose and production of acid or acid+gas

d)

All of the above

22.

The PH indicator used in sugar fermentation test is.............

a)

neutral red

b)

phenol red

c)

phenolphthalein

d)

None of the above

23.

In sugar (Mannitol) fermentation test.......S.aureus is......

a)

Mannitol fermenter (yellow color) with acid+gas production

b)

Mannitol fermenter(red color) with acid production

c)

Mannitol fermenter(yellow color) with acid production

d)

Mannitol fermenter(red color) with acid+gas production

e)

Non mannitol fermenter

24.

In sugar (Mannitol) fermentation test.......S.epidermidis is......

a)

Mannitol fermenter (yellow color) with acid+gas production

b)

Mannitol fermenter(red color) with acid production

c)

Mannitol fermenter(yellow color) with acid production

d)

Mannitol fermenter(red color) with acid+gas production

e)

Non mannitol fermenter(red color)

25.

In sugar (Mannitol) fermentation test.......S.saprophyticus is......

a)

Mannitol fermenter (yellow color) with acid+gas production

b)

Mannitol fermenter(red color) with acid production

c)

Mannitol fermenter(yellow color) with acid production

d)

Mannitol fermenter(red color) with acid+gas production

e)

Non mannitol fermenter(red color)

26.

In sugar (lactose) fermentation test.......E.coli is......

a)

Lactose fermenter (yellow color) with acid+gas production

b)

Lactose fermenter(red color) with acid production

c)

Lactose fermenter(yellow color) with acid production

d)

Lactose fermenter(red color) with acid+gas production

e)

Non lactose fermenter(red color)

27.

In sugar (lactose) fermentation test.......Salmonella is......

a)

Lactose fermenter (yellow color) with acid+gas production

b)

Lactose fermenter(red color) with acid production

c)

Lactose fermenter(yellow color) with acid production

d)

Lactose fermenter(red color) with acid+gas production

e)

Non lactose fermenter(red color)

28.

In sugar (lactose) fermentation test.......Proteus is......

a)

Lactose fermenter (yellow color) with acid+gas production

b)

Lactose fermenter(red color) with acid production

c)

Lactose fermenter(yellow color) with acid production

d)

Lactose fermenter(red color) with acid+gas production

e)

Non lactose fermenter(red color)

29.

In sugar (lactose) fermentation test.......Klebsiella is......

a)

Lactose fermenter (yellow color) with acid+gas production

b)

Lactose fermenter(red color) with acid production

c)

Lactose fermenter(yellow color) with acid production

d)

Lactose fermenter(red color) with acid+gas production

e)

Non lactose fermenter(red color)

30.

In sugar (lactose) fermentation test.....gas production detected by absence or presence of gas in.........

a)

screw capped tube

b)

Durham tube

c)

Test tube

d)

cryo-tube

31.

Litmus milk test used to differentiate Micro-organisms on the basis of...............

a)

Lactose fermentation

b)

renin secretion

c)

casein coagulation

d)

mannitol fermentation

32.

The reagent used in Litmus milk test is........

a)

phenol red

b)

2.5%alcoholic litmus solution

c)

sulfanilic acid

d)

none of the above

33.

The positive result of litmus milk test can appear as.....

a)

pink color

b)

white color

c)

pink color+clot

d)

blue color

e)

violet color

34.

The Negative result of litmus milk test can appear as.....

a)

pink color

b)

white color

c)

pink color+clot

d)

blue color

e)

violet color

35.

Clostridium perfringens in Litmus milk test gives.....

a)

white color

b)

stormy fermentation

c)

pink color

d)

pink color+clot formation

e)

blue color

36.

Clostridium tetani in Litmus milk test gives.....

a)

white color

b)

stormy fermentation

c)

pink color

d)

pink color+clot formation

e)

violet color

37.

Corynebacterium spp. in Litmus milk test gives.....

a)

white color

b)

alkaline reaction

c)

pink color

d)

pink color+clot formation

e)

blue color

38.

Streptococcus pyogens in Litmus milk test gives.....

a)

pink color

b)

alkaline reaction

c)

acid reaction

d)

pink color+clot formation

e)

blue color

39.

Streptococcus agalactiae in Litmus milk test gives.....

a)

pink color

b)

alkaline reaction

c)

acid reaction

d)

pink color+clot formation

e)

violet color

40.

pink color in Litmus milk test indicates.....

a)

acid reaction

b)

alkaline reaction

c)

negative result

d)

Acid, clot, gas and color reduction

e)

acid reaction and clot formation

41.

pink color+clots in Litmus milk test indicates.....

a)

acid reaction

b)

alkaline reaction

c)

negative result

d)

Acid, clot, gas and color reduction

e)

acid reaction and clot formation

42.

blue color in Litmus milk test indicates.....

a)

acid reaction

b)

alkaline reaction

c)

negative result

d)

Acid, clot, gas and color reduction

e)

acid reaction and clot formation

43.

violet color in Litmus milk test indicates.....

a)

acid reaction

b)

alkaline reaction

c)

negative result

d)

Acid, clot, gas and color reduction

e)

acid reaction and clot formation

44.

white color in Litmus milk test indicates.....

a)

acid reaction

b)

alkaline reaction

c)

negative result

d)

Acid, clot, gas and color reduction

e)

acid reaction and clot formation

45.

stormy fermentation in Litmus milk test indicates.....

a)

acid reaction

b)

alkaline reaction

c)

negative result

d)

Acid, clot, gas and color reduction

e)

acid reaction and clot formation

46.

IMViC is abbreviation of ............biochemical tests

a)

3

b)

4

c)

5

d)

none of the above

47.

IMViC is abbreviation of............

a)

indole-mannitol-vogeus proskaur-catalase

b)

indole-methyle red-voges proskaur-citrate

c)

indole-motility test-voges proskaur-coagulase

d)

none of the above

48.

E.coli IMViC tests results are............

a)

+/+/-/-

b)

+/-/+/-

c)

-/-/+/+

d)

-/+/-/+

49.

Salmonella spp IMViC tests results are............

a)

+/+/-/-

b)

+/-/+/-

c)

-/-/+/+

d)

-/+/-/+

50.

Klebsiella spp IMViC tests results are............

a)

+/+/-/-

b)

+/-/+/-

c)

-/-/+/+

d)

-/+/-/+

51.

proteus vulgaris IMViC tests results are............

a)

+/+/-/-

b)

+/-/-/-

c)

-/-/+/+

d)

-/+/-/+

52.

proteus vulgaris IMViC/urease tests results are............

a)

+/+/-/-/-

b)

+/-/-/-/+

c)

-/-/+/+/+

d)

-/+/-/+/-

53.

Klebsiella spp IMViC/urease tests results are............

a)

+/+/-/-/-

b)

+/-/+/-/+

c)

-/-/+/+/+

d)

-/+/-/+/-

54.

Salmonella spp IMViC/urease tests results are............

a)

+/+/-/-/-

b)

+/-/+/-/+

c)

-/-/+/+/+

d)

-/+/-/+/-

55.

E.coli IMViC/urease tests results are............

a)

+/+/-/-/-

b)

+/-/+/-/+

c)

-/-/+/+/+

d)

-/+/-/+/-

56.

select from the following the biochemical tests that involve sugar fermentation

a)

TSI or KIA test

b)

sugar(mannitol or lactose) fermentation test

c)

litmus milk test

d)

methyle red test

e)

voges proskaur test

57.

Citrate test detect ability of bacteria to utilize............as soul source of.......................resulting in...................condition

a)

citrate-nitrogen-alkaline

b)

CHO-phosphorus-acid

c)

citrate-carbon-alkaline

d)

CHO-nitrogen-acid

58.

The name of microbiological media used in citrate test is

a)

mannitol salt agar

b)

muller hinton agar

c)

nutrient agar

d)

Simmon's citrate agar

59.

...........................is the indicator used in citrate test

a)

sulfanilic acid

b)

neutral red

c)

bromothymol blue

d)

phenol red

60.

positive citrate appear as..................

a)

green color

b)

yellow color

c)

pink color

d)

blue color

61.

positive urease appear as..................

a)

green color

b)

yellow color

c)

pink color

d)

blue color

62.

negative urease appear as..................

a)

green color

b)

yellow color

c)

pink color

d)

blue color

63.

negative citrate appear as..................

a)

green color

b)

yellow color

c)

pink color

d)

blue color

64.

which of the following is citrate positive.......

a)

Salmonella typhi

b)

Salmonella typhimurium and other Salmonella spp

c)

E.coli

d)

Klebsiella spp

e)

Proteus spp

65.

which of the following is citrate negative.......

a)

Salmonella typhi

b)

Salmonella typhimurium and other Salmonella spp

c)

E.coli

d)

Klebsiella spp

e)

Proteus spp

66.

which of the following is urease negative.......

a)

Proteus spp

b)

Salmonella spp

c)

E.coli

d)

Klebsiella spp

67.

which of the following is urease positive.......

a)

Proteus spp

b)

Salmonella spp

c)

E.coli

d)

Klebsiella spp

68.

which of the following is indole positive.......

a)

Proteus spp

b)

Salmonella spp

c)

E.coli

d)

Klebsiella spp

69.

which of the following is indole negative.......

a)

Proteus spp

b)

Salmonella spp

c)

E.coli

d)

Klebsiella spp

70.

which of the following is methyle red negative.......

a)

Proteus spp

b)

Salmonella spp

c)

E.coli

d)

Klebsiella spp

71.

which of the following is methyle red positive.......

a)

Proteus spp

b)

Salmonella spp

c)

E.coli

d)

Klebsiella spp

72.

which of the following is voges proskaur positive.......

a)

Proteus spp

b)

Salmonella spp

c)

E.coli

d)

Klebsiella spp

73.

which of the following is voges proskaur negative.......

a)

Proteus spp

b)

Salmonella spp

c)

E.coli

d)

Klebsiella spp

74.

negative indole appear as..................

a)

red ring

b)

colorless ring

c)

pink color

d)

blue color

75.

positive indole appear as..................

a)

red ring

b)

colorless ring

c)

pink color

d)

blue color

76.

positive methyle red appear as..................

a)

red color

b)

yellow color

c)

pink color

d)

blue color

77.

positive voges proskaur appear as..................

a)

red color

b)

yellow color

c)

pink color

d)

blue color

78.

negative voges proskaur appear as..................

a)

red color

b)

yellow color

c)

pink color

d)

blue color

79.

negative methyle red appear as..................

a)

red color

b)

yellow color

c)

pink color

d)

blue color

80.

..............................is the indicator used in urease test

a)

phenol red

b)

neutral red

c)

bromothymol blue

d)

none of the above

81.

phenol red as PH indicator used in.......

a)

Mannitol salt agar(MSA)

b)

urease test

c)

sugar(mannitol or lactose) fermentation test

d)

TSI (triple sugar iron test) or KIA (kligler iron agar)

e)

all of the above

82.

The name of bacteriological media used in urease test is..................................

a)

nutrient agar

b)

Simmon's citrate agar

c)

Christensen's urea medium

d)

muller hinton agar

83.

urease test detect ability of bacteria to secrete urease enzyme to split urea into ammonia

a)

true

b)

false

84.

The name of the indole test reagent is..............

a)

alpha naphthalmyne reagent(B)

b)

sulfanilic acid (reagent A)

c)

Kovac's reagent

85.

The indole test media is..........

a)

Tryptophan agar

b)

Tryptophan soft

c)

Tryptophan broth

86.

The indole test detect the ability of bacteria to split tryptophan producing indole(split indole from tryptophan)

a)

True

b)

False

87.

The indicator used in methyle red test is.........

a)

neutral red

b)

phenol red

c)

methyle red

88.

The indicator used in voges Proskaur test is.........

a)

neutral red

b)

phenol red

c)

methyle red

d)

Barritts vp reagent

e)

3ml 5% alphanaphthol in absolute ethanol+1 ml 40% KOH in distilled water

89.

The methyle red test detect bacterial ability to ferment glucose and produce............

a)

acid

b)

neutral end products

c)

acetoin

90.

The voges proskaur test detect bacterial ability to ferment glucose and produce............

a)

acid

b)

neutral end products

c)

acetoin

91.

In the bile esculin test detect the ability of bacteria to survive bile and utilize esculin to esculitin which gives.....

a)

blue color

b)

red color

c)

black color

92.

In the bile esculin test detect the ability of bacteria to survive bile and utilize esculin to esculitin

a)

True

b)

False

93.

Which of the following is bile esculin positive.......

a)

Enterococcus fecalis

b)

Streptococcus pyogens

c)

Listeria monocytogens

94.

The positive result of bile esculin test appear as.....

a)

yellow color

b)

green color

c)

black color

d)

brown color

95.

The negative result of bile esculin test appear as.....

a)

no change in color

b)

olive green color

c)

black color

d)

brown color

96.

Which of the following is bile esculin negative.......

a)

Enterococcus fecalis

b)

Streptococcus pyogens

c)

Listeria monocytogens

d)

Streptococcus agalactiae

97.

Bacillus subtilis gives.....................in sugar(mannitol) fermentation test

a)

acid (yellow) with gas

b)

acid (yellow) without gas

c)

acid(red) with gas

d)

acid(red) without gas

98.

Bacillus cereus gives.....................in sugar(mannitol) fermentation test

a)

acid (yellow) with gas

b)

acid (yellow) without gas

c)

acid(red) with gas

d)

acid(red) without gas

e)

non mannitol fermenter (red)

99.

Gelatin liquefaction test detect ability of bacteria to secret ........................

a)

Gelatinase

b)

coagulase

c)

urease

d)

oxidase

100.

In Gelatin liquefaction test, positive result appear as..........

a)

liquefied gelatin

b)

clot formation

c)

red ring

d)

purple color

e)

red color

101.

................................is Gelatin liquefaction positive

a)

S.aureus

b)

S.epidermidis

c)

Listeria monocytogens

d)

Bacillus spp

e)

S.saprophyticus

102.

................................is Gelatin liquefaction negative

a)

S.aureus

b)

S.epidermidis

c)

Listeria monocytogens

d)

Bacillus spp

e)

S.saprophyticus

103.

the media used in gelatin liquefaction test is.....

a)

nutrient agar

b)

muller hinton agar

c)

nutrient gelatin

d)

agarose

104.

the gelatin liquefaction incubation period is

a)

7 mins

b)

7 hrs

c)

7 ds

105.

the gelatin liquefaction incubation temperature is..

a)

42C

b)

37C

c)

25C

106.

which of the following is considered as staining category

a)

structural stain

b)

differential stain

c)

simple stain

107.

Loffler's methylene blue, polychrome methylene blue and Leishman's stain are examples of......

a)

structural stain

b)

differential stain

c)

simple stain

108.

Loffler's methylene blue stain used to stain

a)

Streptococcus agalactiae in milk smear

b)

Pasteurella multocida in blood film

c)

Mycobacterium tuberculosis in sputum film

d)

Bacillus anthracis on Blood film

109.

polychrome methylene blue stain used to stain

a)

Streptococcus agalactiae in milk smear

b)

Pasteurella multocida in blood film

c)

Mycobacterium tuberculosis in sputum film

d)

Bacillus anthracis in Blood film

110.

Leishman's stain used to stain

a)

Streptococcus agalactiae in milk smear

b)

Pasteurella multocida in blood film

c)

Mycobacterium tuberculosis in sputum film

d)

Bacillus anthracis in Blood film

111.

Zeihl Neelsen's, modified Zeihl Neelsen's and Gram's technique are examples of.....................

a)

Structural stain

b)

Differential stain

c)

Simple stain

112.

spore stain, flagellar stain and capsular stain are examples of.....................

a)

Structural stain

b)

Differential stain

c)

Simple stain

113.

spore stain as

a)

Malachite green

b)

India ink

c)

negative stain

d)

Leifson stain

114.

capsular stain as

a)

Malachite green

b)

India ink

c)

negative stain

d)

Leifson stain

115.

flagellar stain as

a)

Malachite green

b)

India ink

c)

negative stain

d)

Leifson stain

116.

Bacillus and Clostridum endospores stained using

a)

Malachite green

b)

India ink

c)

negative stain

d)

Leifson stain

117.

Candida capsule stained using

a)

Malachite green

b)

India ink

c)

negative stain

d)

Leifson stain

118.

peritrichous flagella of E.coli stained using

a)

Malachite green

b)

India ink

c)

negative stain

d)

Leifson stain

119.

Zeihl Neelsen's stain used to stain

a)

Streptococcus agalactiae in milk smear

b)

Pasteurella multocida in blood film

c)

Mycobacterium tuberculosis in sputum film

d)

Bacillus anthracis in Blood film

120.

The primary stain in Gram's technique is

a)

Crystal violet

b)

Safranin(diluted carbol fuchsin)

c)

Strong(concentrated) carbol fuchsin

d)

methylene blue

121.

The secondary(counter) stain in Gram's technique is

a)

Crystal violet

b)

Safranin(diluted carbol fuchsin)

c)

Strong(concentrated) carbol fuchsin

d)

methylene blue

122.

The secondary(counter) stain in Zeihl Neelsen's technique is

a)

Crystal violet

b)

Safranin(diluted carbol fuchsin)

c)

Strong(concentrated) carbol fuchsin

d)

methylene blue

123.

The Primary stain in Zeihl Neelsen's technique is

a)

Crystal violet

b)

Safranin(diluted carbol fuchsin)

c)

Strong(concentrated) carbol fuchsin

d)

methylene blue

124.

The Decolorizer in Zeihl Neelsen's technique is

a)

Absolute (100%ethanol) ethyle alcohol

b)

Acetone

c)

3% acid alcohol

d)

97% (ethanol) ethyle alcohol + 3%Hcl (acid)

125.

The Decolorizer in Gram's technique is

a)

Absolute (100%ethanol) ethyle alcohol

b)

Acetone

c)

3% acid alcohol

d)

97% (ethanol) ethyle alcohol + 3%Hcl (acid)

126.

A chemical substance that fixes a dye by compining to that dye forming insoluble compound is..................

a)

primary stain

b)

secondary stain

c)

counter stain

d)

mordent

e)

decolorizer

127.

Lugol's iodine in Gram's technique is considered as.........

a)

primary stain

b)

secondary stain

c)

counter stain

d)

mordent

e)

decolorizer

128.

which of the following is acid fast staining......

a)

Gram's technique

b)

Zeihl Neelsen's technique

c)

modified Zeihl Neelsen's technique

129.

Which of the following is considered components of TSI (triple sugar iron test) media

a)

glucose

b)

sucrose

c)

lactose

d)

dextrose

e)

iron

130.

Which of the following is considered components of KIA (kligler iron agar test) media

a)

glucose

b)

sucrose

c)

lactose

d)

dextrose

e)

iron

131.

TSI test media contain ..............sugars

a)

1

b)

2

c)

3

d)

4

e)

5

132.

KIA test media contain ..............sugars

a)

1

b)

2

c)

3

d)

4

e)

5

133.

the slope of TSI test consist of

a)

butt and slant

b)

reverse and observe

c)

none of the above

134.

Pseudomonas aeuroginosa TSI result is

a)

A/A/+gas/-H2S

b)

K/K/-gas/-H2S

c)

K/A/-gas/+H2S

d)

A/A/-gas/-H2S

135.

Staphylococcus spp TSI result is

a)

A/A/+gas/-H2S

b)

K/K/-gas/-H2S

c)

K/A/-gas/+H2S

d)

A/A/-gas/-H2S

136.

Streptococcus spp TSI result is

a)

A/A/+gas/-H2S

b)

K/K/-gas/-H2S

c)

K/A/-gas/+H2S

d)

A/A/-gas/-H2S

137.

E.coli TSI result is

a)

A/A/+gas/-H2S

b)

K/K/-gas/-H2S

c)

K/A/-gas/+H2S

d)

A/A/-gas/-H2S

138.

Klebsiella spp TSI result is

a)

A/A/+gas/-H2S

b)

K/K/-gas/-H2S

c)

K/A/-gas/+H2S

d)

A/A/-gas/-H2S

139.

proteus spp TSI result is

a)

A/A/+gas/-H2S

b)

K/K/-gas/-H2S

c)

K/A/-gas/+H2S

d)

A/A/-gas/-H2S

140.

Salmonella spp TSI result is

a)

A/A/+gas/-H2S

b)

K/K/-gas/-H2S

c)

K/A/-gas/+H2S

d)

A/A/-gas/-H2S

141.

Citrobacter spp TSI result is

a)

A/A/+gas/-H2S

b)

K/K/-gas/-H2S

c)

K/A/-gas/+H2S

d)

A/A/-gas/-H2S

142.

the PH indicator used in TSI or KIA test is

a)

neutral red

b)

methyle red

c)

phenol red

d)

bromothymol blue

143.

in TSI the A indicates

a)

yellow color

b)

acid production

c)

red color

d)

H2S (hydrogen sulfate) production

e)

black color

144.

in TSI the K indicates

a)

yellow color

b)

alkaline reaction

c)

red color

d)

H2S (hydrogen sulfate) production

e)

black color

145.

in TSI the Black color indicates

a)

acid reaction

b)

alkaline reaction

c)

H2S (hydrogen sulfate) production

146.

in TSI the elevated butt indicates

a)

acid reaction

b)

alkaline reaction

c)

H2S (hydrogen sulfate) production

d)

gas production

147.

the black color in TSI is

a)

H2S

b)

hydrogen sulfide

c)

H2S+iron

d)

iron sulfate

e)

FeSo4

148.

in TSI.....

a)

the slant is stabbed and the butt is streaked

b)

the slant is streaked and the butt is stabbed

149.

we can detect ability of bacteria to ferment(3) sugars in(TSI) or (2) sugars in (KIA) and acid, gas and H2S production

a)

True

b)

False

150.

Nitrate reduction test detect ability of bacteria to reduce nitrate to nitrite

a)

True

b)

False

151.

Nitrate reduction positive result appear as

a)

red ring

b)

colorless ring

c)

green color

d)

purple color

152.

Nitrate reduction negative result appear as

a)

red ring

b)

colorless ring

c)

green color

d)

purple color

153.

Nitrate reduction reagents are

a)

reagent A

b)

sulfanilic acid

c)

reagent B

d)

alpha naphthyl amine

154.

Nitrate reduction reagent A is

a)

alpha naphthyl amine

b)

kovac's reagent

c)

sulfanilic acid

d)

bromothymol blue

155.

Nitrate reduction reagent B is

a)

alpha naphthyl amine

b)

kovac's reagent

c)

sulfanilic acid

d)

bromothymol blue

156.

critical step during application of Gram's technique is

a)

primary stain

b)

secondary(counter) stain

c)

decolorizer(ethanol) application

d)

mordent

157.

acid fast micro-organisms means

a)

M.O. moving very fast

b)

can be killed rapidly by antibiotic

c)

resist decolorization by acid alcohol

d)

all of the above

e)

none of the above

158.

API system is abbreviation of Analytical Profile Index

a)

True

b)

False

159.

The results of nitrate reduction test, indole test and oxidase test can be read within...........after specific reagent is added

a)

1-2 ds

b)

1-2 hrs

c)

1-2 mins

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