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WorksheetsBiology Honors-CHAPTER 12 (Modules 12.1-12.5: Gene Tech)
Total questions: 18
Worksheet time: 11mins
Biotechnology
Manipulation of genes for practical purposes
Manipulation of organisms or their components to make useful products
A gene of interest which is copied using DNA technology
Using modering technology to help benefit the environment
How is gene cloning possible?
Scientists are using modern laboratory techniques used to studying and manipulating genetic material (DNA Technology)
Manipulating the mitochondria in order to produce ATP faster so the cell can divide rapidly
Manipulating the enzymes that produces polypeptides
Plasmids...
Small circular DNA found in eukaryotic cells
Have bacterial enzymes that cut at specific sequences
Can be transferred into bacterial cells
Small circular DNA found in bacterial cells
Which of the following statements about Recombinant DNA is false?
Formed when 2 different sources of DNA is combined
Recombinant DNA technology is used today for genetic engineering
Recombinant DNA re-joined together by the same strand
What is the FIRST 3 STEPS of gene cloning?
Transformation occurs, gene of interest is isolated, gene V is activated
DNA ligase is added to join plasmid and gene of interest after plasmid and gene of interest is isolated
Gene of interest is cut, protein is deactivated, RNA ligase is activated
Plasmid is isolated from Vector, gene of interest is isolated, plasmid is cut using a restriction enzyme
What are the NEXT 3 STEPS of gene cloning AFTER STEPS 1-3?
Gene of interest is cut using the restriction enzyme, DNA ligase is added to join plasmid and gene of interest, cut plasmid and cut gene of interest are mixed
Recombinant bacterium replicates to form a clone of cells and gene cloning can be used for immediate product and protein from gene
Gene of interest is cut using the restriction enzyme, cut plasmid and cut gene of interest are mixed, DNA ligase is added to join plasmid and gene of interest
DNA ligase is added to join plasmid and gene of interest, Gene of interest is cut using the restriction enzyme, cut plasmid and cut gene of interest are mixed
What are the FINAL STEPS of gene cloning?
Recombinant plasmid and bacteria are mixed and plasmid undergoes transformation, recombinant bacteria replicates to form a clone of cells which can be used for immediate product or for a protein
Recombinant bacteria replicates to form a clone of cells which can be used for immediate product or for a protein, recombinant plasmid and bacteria are mixed and plasmid undergoes transformation
Restriction enzymes...
Bacterial enzymes that cut DNA at specific sequences
that biologists discovered have hundreds of different restriction enzymes
Are basically molecular scissors
All following choices are true
A restriction site is also known as a _____________.
cut site
repairing site
nucleus
None of the above
Which of the following statements of restriction sites is true?
It has a 4-8 nucleotide pair sequence that restriction enzyme recognizes and is unique to each restriction enzyme.
It cuts parts of the RNA randomly to be spliced into new coding sections
It has a 4-8 nucleotide pair sequence that restriction enzyme recognizes and is the same to each restriction enzyme.
It cuts parts of the DNA randomly to be spliced into new coding sections
Restriction fragments...
Restriction the process of transcription to make RNA
Pieces of DNA and sticky ends that are not complementary to the same restriction enzyme
Pieces of DNA left by restriction enzymes cuts and sticky ends that are complementary to the same restriction enzyme
Pastes DNA fragments together
True or False:
Sticky ends are stranded ends of RNA fragments that can hydrogen bond to a complementary single strand
True
False
Nucleic acid probes...
Both choices are incorrect
Single stranded molecule of fluorescently labeled DNA or RNA and can be short or long
Can be used in DNA microarrays and used to identify probes in bacterial colonies
Why is the use of viral enzyme critical to producing cDNAs?
It uses reverse transcriptase that can produce DNA from RNA
The enzyme and DNA are complementary
It can copy certain genes from DNA in order to make it complementary to cDNAs
mRNA is not complementary is cDNA
What type of RNA makes reverse transcriptase possible?
rRNA
tRNA
mRNA
What is DNA polymerase's necessity in creating cDNA?
It's an enzyme that breaks down mRNA
It is added to make a second DNA strand
It allows reverse transcriptase to occur
It disables certain genes to make sure DNA is synthesized properly and correctly.
What are the CRISPR-Cas9 system functions?
A defense alert in a cell to destroy foreign substances the cell does not recognize
Only disable genes that mutate gene function in cells
Copies genes at a rapid pace
Knocks out or disable genes to study function in cells and edit genes to fix mutations
What is the difference between gene cloning and the CRISPR-Cas9 system?
Gene cloning copies only one gene while CRISPR-Cas9 copies all genes in a cell
They both have the same functions
Gene cloning exactly copies a gene, while the CRISPR-Cas9 system modifies the gene.
Gene cloning allows only a few gene expression while the CRISPR-Cas9 system express all the genes in a cell
