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Biology Honors-CHAPTER 12 (Modules 12.1-12.5: Gene Tech)

Total questions: 18

Worksheet time: 11mins

Name
Class
Date
1.

Biotechnology

a)

Manipulation of genes for practical purposes

b)

Manipulation of organisms or their components to make useful products

c)

A gene of interest which is copied using DNA technology

d)

Using modering technology to help benefit the environment

2.

How is gene cloning possible?

a)

Scientists are using modern laboratory techniques used to studying and manipulating genetic material (DNA Technology)

b)

Manipulating the mitochondria in order to produce ATP faster so the cell can divide rapidly

c)

Manipulating the enzymes that produces polypeptides

3.

Plasmids...

a)

Small circular DNA found in eukaryotic cells

b)

Have bacterial enzymes that cut at specific sequences

c)

Can be transferred into bacterial cells

d)

Small circular DNA found in bacterial cells

4.

Which of the following statements about Recombinant DNA is false?

a)

Formed when 2 different sources of DNA is combined

b)

Recombinant DNA technology is used today for genetic engineering

c)

Recombinant DNA re-joined together by the same strand

5.

What is the FIRST 3 STEPS of gene cloning?

a)

Transformation occurs, gene of interest is isolated, gene V is activated

b)

DNA ligase is added to join plasmid and gene of interest after plasmid and gene of interest is isolated

c)

Gene of interest is cut, protein is deactivated, RNA ligase is activated

d)

Plasmid is isolated from Vector, gene of interest is isolated, plasmid is cut using a restriction enzyme

6.

What are the NEXT 3 STEPS of gene cloning AFTER STEPS 1-3?

a)

Gene of interest is cut using the restriction enzyme, DNA ligase is added to join plasmid and gene of interest, cut plasmid and cut gene of interest are mixed

b)

Recombinant bacterium replicates to form a clone of cells and gene cloning can be used for immediate product and protein from gene

c)

Gene of interest is cut using the restriction enzyme, cut plasmid and cut gene of interest are mixed, DNA ligase is added to join plasmid and gene of interest

d)

DNA ligase is added to join plasmid and gene of interest, Gene of interest is cut using the restriction enzyme, cut plasmid and cut gene of interest are mixed

7.

What are the FINAL STEPS of gene cloning?

a)

Recombinant plasmid and bacteria are mixed and plasmid undergoes transformation, recombinant bacteria replicates to form a clone of cells which can be used for immediate product or for a protein

b)

Recombinant bacteria replicates to form a clone of cells which can be used for immediate product or for a protein, recombinant plasmid and bacteria are mixed and plasmid undergoes transformation

8.

Restriction enzymes...

a)

Bacterial enzymes that cut DNA at specific sequences

b)

that biologists discovered have hundreds of different restriction enzymes

c)

Are basically molecular scissors

d)

All following choices are true

9.

A restriction site is also known as a _____________.

a)

cut site

b)

repairing site

c)

nucleus

d)

None of the above

10.

Which of the following statements of restriction sites is true?

a)

It has a 4-8 nucleotide pair sequence that restriction enzyme recognizes and is unique to each restriction enzyme.

b)

It cuts parts of the RNA randomly to be spliced into new coding sections

c)

It has a 4-8 nucleotide pair sequence that restriction enzyme recognizes and is the same to each restriction enzyme.

d)

It cuts parts of the DNA randomly to be spliced into new coding sections

11.

Restriction fragments...

a)

Restriction the process of transcription to make RNA

b)

Pieces of DNA and sticky ends that are not complementary to the same restriction enzyme

c)

Pieces of DNA left by restriction enzymes cuts and sticky ends that are complementary to the same restriction enzyme

d)

Pastes DNA fragments together

12.

True or False:

Sticky ends are stranded ends of RNA fragments that can hydrogen bond to a complementary single strand

a)

True

b)

False

13.

Nucleic acid probes...

a)

Both choices are incorrect

b)

Single stranded molecule of fluorescently labeled DNA or RNA and can be short or long

c)

Can be used in DNA microarrays and used to identify probes in bacterial colonies

14.

Why is the use of viral enzyme critical to producing cDNAs?

a)

It uses reverse transcriptase that can produce DNA from RNA

b)

The enzyme and DNA are complementary

c)

It can copy certain genes from DNA in order to make it complementary to cDNAs

d)

mRNA is not complementary is cDNA

15.

What type of RNA makes reverse transcriptase possible?

a)

rRNA

b)

tRNA

c)

mRNA

16.

What is DNA polymerase's necessity in creating cDNA?

a)

It's an enzyme that breaks down mRNA

b)

It is added to make a second DNA strand

c)

It allows reverse transcriptase to occur

d)

It disables certain genes to make sure DNA is synthesized properly and correctly.

17.

What are the CRISPR-Cas9 system functions?

a)

A defense alert in a cell to destroy foreign substances the cell does not recognize

b)

Only disable genes that mutate gene function in cells

c)

Copies genes at a rapid pace

d)

Knocks out or disable genes to study function in cells and edit genes to fix mutations

18.

What is the difference between gene cloning and the CRISPR-Cas9 system?

a)

Gene cloning copies only one gene while CRISPR-Cas9 copies all genes in a cell

b)

They both have the same functions

c)

Gene cloning exactly copies a gene, while the CRISPR-Cas9 system modifies the gene.

d)

Gene cloning allows only a few gene expression while the CRISPR-Cas9 system express all the genes in a cell