WorksheetsSDS-PAGE
Total questions: 11
Worksheet time: 6mins
The matrix used in SDS-PAGE is the same material used for common agarose gel for DNA separation
True
False
The gel-position for agarose-gel and SDS-PAGE is horizontal and vertical, respectively.
True
False
In agarose gel, bands move to +ve changed electrode. However, in SDS-PAGE, bands move to -ve charged electrode.
True
False
Sodium Dodecyl Sulfate is a chemical that can reduce S=S bond
True
False
SDS-PAGE can be used to determine protein purity of a given sample
True
False
A heterodimer enzyme-X consists of a subunit of 20kD and a subunit of 35 kDa. What is the size of band that will appear on modified SDS-PAGE (without Beta-mercaptoethanol treatment)?
two bands of 20kD and 35kD, respectively
One band with 55 kDa
Three bands with 20kD, 35kD, and 55 kDa, respectively
Smear because separation will be poor
A complex enzyme-S consists of two subunits of 10kD each, and two subunits of 20 kDa each. What is the size of band that will appear on SDS-PAGE (with Beta-mercaptoethanol treatment)?
bands with 10kD and 20kD, respectively
bands with 20kD and 40kD, respectively
One band with 30 kDa
One band with 60 kDa
After electrophoresis has completed, the band will appear after straining in Coomassie blue solution.
True
False
After breaking a culture of E. coli, the extracted protein is run in a SDS-PAGE. Guess what will you see?
Single band
Multiple bands
Coomassie blue has another name. It is....
Smart Blue
Brilliant Blue
Superb Blue
Coomassie blue is a common stain for SDS-PAGE. However, the detection sensitivity is low. A better alternative staining approach is:
ethidium bromide
silver nitrate
gram stain
