Font size
WorksheetsCloning Vectors
Total questions: 20
Worksheet time: 14mins
Some restriction enzymes produce "sticky ends". Sticky ends are . . .
single-stranded DNA "overhangs" that can bind to complementary DNA
double stranded DNA aligned with each other
single-stranded DNA aligned with host DNA
ends of a double stranded DNA strand
How do we name restriction Enzymes ?
First letter in Cap for Genus, next two letter in small for species, next one letter for strain and roman numeral for order of discovery
First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and English numeral for order of discovery
First two letter in Cap for Genus, next one letter in capital for species, next one letter for strain and roman numeral for order of discovery
First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and roman numeral for order of discovery
Which RE's from above make blunt cuts? (More than one answer)
Alul
HaeIII
BamHI
HindIII
EcoRI
Which RE's from above make sticky cuts? (More than one answer)
Alul
HaeIII
BamHI
HindIII
EcoRI
Restriction Enzymes were primarily used as....
defense by protozoa against antibiotics
tools by viruses to cleave bacterial genome
defense by bacteria against bacteriophages viruses
tools to cleave DNA during replication
What is a palindrome?
A sticky end resulting from a restriction enzyme
A blunt end resulting from a restriction enzyme
A sequence that reads the same forwards and backwards
A sequence that is complementary to the other
Identify the given plasmid.
pUC19
pBR322
pUC119
pUC18
What are selectable marker ?
a radium labelled marker
a type of sequence
resistant gene
a type of protein
LacZ gene is used to?
Differentiate between transformant and non transformant
Differentiate between clones with vector plasmids and without vector plasmids
Differentiate between competent cells and non competent cells
Differntiate between negative and false negative
What is the origin of replication ?
place of origin
sequence of genome where replication starts
where DNA are formed
where RNA are formed
What is transformation?
Procedure though which a piece of protein incorporated into bacterium
Procedure though which a piece of lipid incorporated into bacterium
Procedure though which a piece of DNA incorporated into bacterium
Procedure though which a piece of carbohydrate incorporated into bacterium
In order to insert a human gene into plasmid, both must
code for the same gene product.
be cut by the same restriction enzyme.
originate from the same type of cell.
have identical sequences.
The alternative selectable marker _______________ and chromogenic substrate __________________ that selects recombinants on the basis of their ability to produce colour are
LacZ gene , X-gal
α-galactosidase and X-gal
Amp R gene and X-gal
β-galactosidase and X-gal
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
Insertional inactivation of β-galactosidase gene in recombinant bacteria
Inactivation of glycosidase gene in recombinant bacteria
Incompetence of recombinant bacteria containing β-galactosidase
insertional inactivation of α-galactosidase gene in non-recombinant bacteria
Non essential genes/stuffer fragments are removed from bacteriophage because
To increase the phage genomic DNA size
To reduce the phage genomic RNA size
To fit in other phage DNA
To fit in the foreign gene to be cloned
E.coli DH5alpha is made chemically competent by
giving them a freeze shock
giving them a heat shock
giving them electrical current
all of above
What is the importance of cos sites in cosmids?
to make cosmids infectious
to make cosmids competent
allow DNA to be packaged inside lambda phage heads
permit replication as plasmid in bacteria
How do you overcome vector religation problem?
Use ligase enzyme
Use alkaline phosphatase enzyme
Use restriction enzymes
Use polymerase enzyme
