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Worksheetsfinal res enz
Total questions: 15
Worksheet time: 8mins
Retriction enzymes bind to DNA in which form
monomer
dimer
trimer
none
Restriction enzyme cut the sugar phosphate backbone by mechanism of
phosphorylation
hydrolysis
esterification
catenation
isoschizomers are different restriction enzyme with
same recognition site and same cleavage point
same recognition site and different cleavage point
both
none
Sma I is a
blunt end cutter
cohesive end cutter
both
none
restriction enzyme require as a co factor
Zn
Ca
Mg
Mn
neoschizomers are restiction enzymes with
same recognition and cleavage site
same recognition but different cleavage site
different recognition and different cleavage site
none
for cloning a DNA fragment into a vector both should be cut ideally with
same restriction enzyme
different restriction enzyme
both
none
Restriction enzymes are part of
phosphorylase enzyme system
Restriction modification system
All of the above
None of the above
Type of restriction enzyme used in gene cloning
Type 1
Type 2
Type 3
None
In EcoRI R stands for
Species
Genus
Strain
None
Type 2 restriction enzyme cuts
Far away from the site
Within the site
Near the site
None
Restriction enzyme cleave the
Hydrogen bond
Phospho-diester bond
Both
None
Bam HI is
Cohesive cutter
blunt end cutter
Both
None
ligation is easy to perform with DNA having what type of end
blunt end
cohesive end
both
none
Bam HI and Sau3A1 are
neoschizomers
isoschizomers
both
none
