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WorksheetsExpression of Biological Information
Total questions: 50
Worksheet time: 17mins
The Central Dogma of Biology stated as
Protein→RNA→DNA
RNA→DNA→protein
DNA→protein→RNA
DNA→RNA→protein
State the model used in DNA replication
Dispersive
Conservative
Semi-conservative
When does DNA replication occur?
S phase of interphase
Transcription process
G1 phase of interphase
Elongation phase
Which of these proteins involved in DNA replication?
Single-stranded binding protein
Helicase
RNA polymerase
Promoter
Topoisomerase
Name the strand that replicated continuously toward replication fork
Okazaki Fragment
Lagging strand
Leading strand
Antisense strand
Name the bond formed by DNA ligase
phosphorus bond
peptide bond
ester bond
phosphodiester bond
State the direction of new strand formation in DNA replication
5'→3'
3'→5'
Name the strand that been used by DNA polymerase or RNA polymerase to attach complementary bases during DNA replication or Transcription
(a)
Which experiment proved that DNA replication is semiconservative replication?
Experiment of Beadle and Tatum
Experiment Meselson and Stahl
Experiment of Harshey an Chase
Experiment of Avert, MacLeod and McCarty
How absence of topoisomerase affect DNA replication
Supercoiling of DNA
Hydrogen bond reformed between the strand
Unwinding of DNA strand stopped
Phosphodiester bond break
Name the protein that remove the RNA primer and replace with DNA nucleotides
Primase
DNA Polymerase I
DNA Polymerase III
In DNA replication,name the fragments synthesized discontinuously away from replication fork
(a)
Where does DNA replication occur
Nucleus
Nucleolus
Cytoplasm
After DNA replication is completed
each new DNA double helix consists of two new strands
one DNA double helix consists of two old strands and one DNA double helix consists of two new strands.
each new DNA double helix consists of one old DNA strand and one new DNA strand
Each of the four DNA strands consists of some old strand parts and some new strands parts.
Where does RNA splicing occur?
Cytoplasm
Nucleus
Nucleolus
How does template is read during transcription process?
Template is read from 3' end to 5' end
Template is read from 5' end to 3' end
Template is read in both direction
Template is read alternately
How many nucleotide present in a codon?
4
3
2
1
Where does Translation process occur
Nucleus
Cytoplasm
Nucleolus
State the enzyme that initiates Transcription process
Helicase
DNA polymerase
RNA polymerase
Primase
Name the enzyme that catalysed the attachment of amino acid to tRNA
RNA polymerase
Promoter
Terminator
aminoacyl-tRNA synthetase
One tRNA carry only one specific amino acid. So how many tRNA do we have?
4
20
10
5
Eukaryotic mRNA molecules are occasionally interspersed with non-coding sequences. They are called
Anticodon
Introns
Exons
Codon
State the function of P site of ribosomes
holds the tRNA carrying the growing polypeptide chain
holds the tRnA carrying the next amino acid to be added
discharged tRNA leaves the ribosome
If the DNA triplet code were ATG CGT, the tRNA anticodons would be
AUG CGU
ATG CGT
UAG GCA
UAG CGU
What codon code for Methionine?
3'-AUG-5'
3'-UAG-5'
3'-GUA-5'
3'-AGU-5'
Which of these are STOP codon?
UGG
UAG
UAA
UGA
State the steps involved in Elongation stage of Translation process
Termination
Codon recognition
Promoter binding
Peptide bond formation
Translocation
Name the site for RNA polymerase to bind on mRNA during transcription process
Terminator
Promoter
Operator
Repressor
Which of these steps in Transcription process?
Initiation
Codon recognition
Termination
Peptide bond formation
Elongation
What happen when ribosome reaches a stop codon on mRNA
Peptide bond between amino acids is hydrolyzes by release factor
Bond between the last amino acid and tRNA in P site is hydrolyzes by release factor
Bond between first amino acid and tRNA in A site is hydrolyzes by release factor
Bond between anticodon of tRNA and codon mRNA is hydrolyzes by release factor
A structure of many ribosomes attach to a single mRNA is known as
Multiribosomes
Polyribosomals
Triribosomes
Polyribosomes
lac operon is one example operon in bacteria. Name the genes in the operon
lac I
lac Z
lac Y
lac A
E.coli uses 3 enzymes to absorb and metabolize lactose. Name the three enzymes
Galactase
β-galactosidase
Permease
Lactase
Transacetylase
Name the gene codes for Transacetylase
lac I
lac Z
lac Y
lac A
Which of these protein help to transport lactose into the cell of bacteria
β-galactosidase
Transacetylase
Permease
Amilase
Name the protein produced by lac I
Repressor protein
Operator
Permease
Promoter
What happen when repressor protein is activated
Transcription occur and mRNA is produced
Translation occur and protein is produced
Transcription and translation is stopped
Transcription occur but translation is stopped
What happen when lactose is present?
Lactose is converted to its isomer, allolactose
Repressor protein bind to operator
RNA polymerase bind to promoter
The operon is 'switched on'
Where does lac operon can be found?
Bacteria nucleus
Bacteria mitochondrion
Bacteria plasmid
Bacteria ribosome
State the function of β-galactosidase
Detoxifies other molecules entering the cell
Transports lactose into the cell
Transfer an acetyl group from acetyl-CoA
Hydrolyses lactose to glucose and galactose
Name the above complex
(a)
Based in the diagram,state the anticodon found on the tRNA above
3'-TAC-5'
3'-UTC-5'
3'-UAC-5'
Based on diagram above, name the chemical bond formed between the amino acids
Hydrogen bond
Phosphodiester bond
Glycosidic bind
Peptide bond
What stage of Transcription shown in above diagram
Initiation
Elongation
Termination
Name protein A
Helicase
DNA polymerase III
Primase
DNA ligase
State the function of protein Z
Unwind the parental double helix at replication fork
Relieve the strain due to unwinding process
Bind nd stabilize single-stranded DNA until i can be used as a template
Joined/anneal the Okazaki fragments
Which of these statements is true
Transcription of the 3 structural genes cannot occur
The 3 enzymes able to synthesized
The operon is switched off
Lactose is broken down into glucose and galactose
State the importance of DNA replication in S phase
To increase the number of organelles
To maintain energy storage
To maintain chromosome number and composition in daughter cell
To increase the amount of DNA
Does RNA splicing happen in prokaryotic cell?
Yes
No
What happen to the Ribosome when it reach to stop codon?
The two ribosomal subunit disintegrate
The two ribosomal subunit dissociate
The two ribosomal hydrolyzed by release factor
The two ribosomal subunit autolysis
