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Microbiological Culture and Testing

Total questions: 80

Worksheet time: 43mins

Name
Class
Date
1.

Which structure does bacteria NOT have?

a)

Flagellum

b)

Nucleus

c)

Cell Wall

d)

Cell Membrane

2.

Which organisms is larger?

a)

Virus

b)

Bacteria

3.

single celled organism that lacks a nucleus

a)

bacteria

b)

cell wall

c)

flagellum

d)

endospore

4.

a long, whip-like structure that helps a cell to move.

a)

pasteurization

b)

bacteria

c)

flagellum

d)

endospore

5.

What shape are these bacteria?

a)

cocci

b)

bacili

c)

spirilla

d)

linguine

6.

What shape are these bacteria?

a)

cocci

b)

bacilli

c)

spirilla

d)

fusilli

7.

What shape are these bacteria?

a)

cocci

b)

bacilli

c)

spirilla

d)

tortellini

8.

Cells that lack membrane bound organelles such a nucleus are called

a)

prokaryotic

b)

eukaryotic

9.
Bacteria have a cell membrane
a)
True
b)
False
c)
Some do, some don't
d)
Only in certain temperature conditions
10.
Bacteria cells have all of the following EXCEPT which?
a)
mitochondria
b)
genetic material
c)
cell membrane
d)
ribosomes
11.
A structure that can help bacteria survive harsh conditions.
a)
capsids
b)
endospores
c)
pili
d)
DNA
12.
Which organism can survive either with or without oxygen?
a)
heterotrophs
b)
autotrophs
c)
obligate anaerobe
d)
facultative anaerobe
13.
Bacteria exchange genetic information during 
a)
asexual reproduction
b)
binary fission
c)
conjugation
d)
photosynthesis
14.
Photoheterotrophs will use 
a)
oxygen or carbon dioxide for energy
b)
nitrogen or peptidoglycan for energy
c)
Organic compounds or light
d)
Chemicals or light
15.
Which organism cannot survive in the presence of oxygen?
a)
heterotrophs
b)
autotrophs
c)
obligate anaerobe
d)
facultative anaerobe
16.
During an experiment a scientist observed prokaryotes that lived near volcanic vents deep in the ocean.  The scientist must likely observed
a)
photoautotrophs
b)
chemoautotrophs 
c)
heterotrophs
d)
photoheterotrophs
17.

The outer covering of ALL bacteria cells is called the

a)

flagella

b)

cytoplasm

c)

cell wall

d)

capsule

18.
Live bacteria are found in milk, cheese and yogurt
a)
True
b)
False
19.

This type of complex media is more rich than nutrient agar and includes the nutrient hemin.

a)

chocolate agar

b)

blood agar

c)

LB agar

d)

MacConkey agar

20.

This type of complex media contains lysed red blood cells and additional nutrients.

a)

blood agar

b)

MacConkey agar

c)

nutrient agar

d)

chocolate agar

21.

This type of media inhibits the growth of organisms other than the one being grown. Can be complex or chemically defined media.

a)

selective media

b)

diferential media

c)

chemically defined media

d)

enrichment culture media

22.

This type of media contains specific ingredients that help us to visually distinguish which species do or do not carry out a specific biochemical process.

a)

selective media

b)

differential media

c)

chemically defined media

d)

enrichment culture media

23.

T/F: media can be both selective and differential.

a)

True

b)

False

24.

This media selects for gram-negative organisms and isolates lactose fermenters (pink) and non-lactose fermenters (clear).

a)

choclate agar

b)

Mannitol salt agar

c)

Eosin-methylene blue (EMB) agar

d)

MacConkey agar

25.

This is a general purpose medium that supports the growth of a wide variety of bacteria.

a)

blood agar

b)

nutrient agar

c)

chocolate agar

d)

MacConkey agar

26.

The substance in nutrient agar that makes the nutrient agar a solid is

a)

casein

b)

agar

c)

water

d)

meat extract

27.

What is the difference between nutrient agar and lactose broth?

a)

Nutrient agar is solid and lactose broth is liquid

b)

Both are liquid but lactose broth has added lactose sugar

c)

Nutrient agar is a selective media and lactose broth is a derived media

d)

there is no difference between the two types

28.

Nutrient broth is turned into nutrient agar by the addition of what?

a)

lactose

b)

eggs

c)

agar

d)

peptone

29.

The type of culture media that can turn bacteria different colors on a petri dish is called a ____ media

a)

selective

b)

differential

c)

natural

d)

broth

30.

Regardless of the media type, the best temperature for most bacteria is

a)

10 -15o C

b)

25 - 40oC

c)

60oC

d)

100oC

31.

What is the difference between nutrient agar and nutrient broth.

a)

one is liquid and one is solid

b)

one is sterile and one is not sterile

c)

one is acidic and one is basic

d)

one has extra sugar and one does not

32.
Plates should be incubated
a)
agar side up
b)
agar side down 
c)
it doesn't matter
33.
The area where bacteria cannot survive because of the antibiotic:
a)
zone of inhibition
b)
zone of prohibition
c)
the friend zone
d)
zone of unhappiness
34.

How many antibiotics does this bacteria seem to be resistant to?

a)

2

b)

3

c)

All of them

d)

None of them

35.
Based on the figure, which antibiotic inhibited the growth of the bacteria the most?
a)
A
b)
B
c)
C
d)
D
36.

A student is assigned to measure the susceptibility of a strain of bacteria to 3 different antibiotics in Biology class. The student first divides the agar plate into four different quadrants, one of which is reserved for a Control. Which of the following best describes the purpose of the Control in this experiment?

a)

The Control quadrant is used to grow a different strain of bacteria so that you can compare it with the one you are testing.

b)

The Control quadrant helps demonstrate whether or not the same strain of bacteria grew correctly on the plate in the absence of any antibiotic.

c)

The Control quadrant contains all three antibiotics to show what completely inhibited growth would look like.

d)

The Control quadrant is the only quadrant on which no bacteria is grown and so that none of the bacteria comes in direct contact with the antibiotic.

37.

Select the example of an antibiotic.

a)

vaccine

b)

pathogen

c)

penicillin

d)

antibody

38.
Antibiotics kill viruses
a)
True
b)
False
39.

What is present in the endospore

a)

DNA only

b)

DNA and specific proteins

c)

specific proteins only

d)

the entire cell

40.

What is non selective, contains all elements needed for growth of bacteria?

a)

medium

b)

primary media

c)

indicator media

d)

selective media

e)

transport media

41.

What is a defined mass of bacteria assumed to have grown from a single organism?

a)

fission

b)

bacterial conjugation

c)

progeny

d)

colony

42.

What is growth media that used for strict anaerobes?

a)

agar

b)

eosin methylene blue agar

c)

thioglycollate broth

d)

macconkey agar

43.

What is an agar that is used for the routine cultivation of non-fastidious bacteria?

a)

nutrient agar

b)

blood agar

c)

ssa agar

d)

chocolate agar

e)

thayer-martin chocolate agar

44.

What is the act of eliminating all the living microorganisms from an article or area?

a)

antiseptic

b)

disinfectant

c)

sterilization

d)

vaccine

45.

What is the reproductive process in which the parent cell divides into two identical independent cells?

a)

fission

b)

bacterial conjugation

c)

progeny

d)

colony

46.

What is a substance that fixes a dye or stain to an object?

a)

mordant

b)

counter stain

c)

catalase test

d)

fecal occult test

e)

immunoassay

47.

All of the following are true concerning aseptic technique EXCEPT:

a)

it prevents bacteria from infecting humans

b)

it prevents contamination of a culture by unwanted organisms

c)

it prevents contamination of surfaces

d)

it includes only physical means of prevention

48.

Which bacterial structure helps the bacteria transfer plasmids during bacterial conjugation?

a)

plasma membrane

b)

capsule

c)

fimbrae

d)

pili

e)

cell wall

49.

Which group of organisms can be multicellular?

a)

prion

b)

eukaryotes

c)

prokaryotes

d)

viruses

50.

Which bacterial structure helps the bacteria bind to surfaces and evade phagocytosis?

a)

plasma membrane

b)

capsule

c)

fimbrae

d)

pili

e)

cell wall

51.

What is used as temporary storage, prevents microbial multiplication?

a)

medium

b)

primary media

c)

indicator media

d)

selective media

e)

transport media

52.

What is an agar that differentiates salmonella and shigella colonies from other enterobacteriaceae; obtained from stool samples?

a)

nutrient agar

b)

blood agar

c)

ssa agar

d)

chocolate agar

e)

thayer-martin chocolate agar

53.

What type of bacteria do endospores form?

a)

gram positive bacillus

b)

gram negative bacillus

c)

gram positive coccus

d)

gram negative coccus

54.

Which of the following is NOT a morphological type of bacteria?

a)

coccus

b)

bacillus

c)

coccobacillus

d)

none of the above

55.

What is an agar that is useful for cultivating fastidious organisms and for determining the hemolytic capabilities of an organism (used in strep tests)?

a)

nutrient agar

b)

blood agar

c)

ssa agar

d)

chocolate agar

e)

thayer-martin chocolate agar

56.

What are microorganisms that love salt?

a)

fastidious bacteria

b)

methanogens

c)

halophiles

d)

thermophiles

e)

cyanobacteria

57.

What is a chemical used on living tissues to control the growth of infectious agents?

a)

antiseptic

b)

disinfectant

c)

sterilization

d)

vaccine

58.

What is a general term for organisms that cannot produce their own food and must consume other organisms?

a)

autotrophs

b)

heterotrophs

c)

photosynthetic

d)

chemosynthetic

e)

saprobes

59.

What is a dye that adds a contrasting color?

a)

mordant

b)

counter stain

c)

catalase test

d)

fecal occult test

e)

immunoassay

60.

What is the test that detects the presence of specific enzyme by adding hydrogen peroxide?

a)

mordant

b)

counter stain

c)

catalase test

d)

fecal occult test

e)

immunoassay

61.

This bacterial growth curve is used to show on bacteria grow in liquid culture or on a bacterial growth plate. Which is the location on the graph where there is a high amount of excess waste and extremely low nutrients?

a)

a

b)

b

c)

c

d)

d

62.

What is a type of staining that uses a mordant of steam?

a)

gram staining

b)

endospore staining

c)

simple staining

d)

steam staining

63.

What are bacteria that has a complex nutritional requirement?

a)

fastidious bacteria

b)

methanogens

c)

halophiles

d)

thermophiles

e)

cyanobacteria

64.

Which bacterial structure helps the bacteria connect to each other and helps the pathogenic bacteria cause disease?

a)

plasma membrane

b)

capsule

c)

fimbrae

d)

pili

e)

cell wall

65.

Which of the following classification(s) of bacteria is/are related to high pH?

a)

acidophiles

b)

alkalophiles

c)

neutrophiles

d)

all of the above

e)

none of the above

c. neutrophiles

66.

This bacterial growth curve is used to show on bacteria grow in liquid culture or on a bacterial growth plate. Which is the location on the graph where the first endospores would start to form?

a)

a

b)

b

c)

c

d)

d

67.

What is the difference in the bacteria that leads to gram positive?

a)

thicker layer of peptidoglycan

b)

thicker layer of phosopholipids

c)

thinner layer of peptidoglycan

d)

thicker nuclear membrane

68.

What is an agar is used exclusively for growing vibrio bacteria such as Vibrio cholerae?

a)

TCBS

b)

blood agar

c)

ssa agar

d)

chocolate agar

e)

thayer-martin chocolate agar

69.

______ is used to determine if a bacteria has polysaccharide layer that lies outside the cell wall of the bacteria.

a)

negative staining

b)

endospore staining

c)

capsule staining

d)

gram staining

70.

What are microorganisms that love heat?

a)

fastidious bacteria

b)

methanogens

c)

halophiles

d)

thermophiles

e)

cyanobacteria

71.

Which of the following classification(s) of bacteria is/are related to temperature?

a)

psychrophiles

b)

mesophiles

c)

extreme thermophiles

d)

all of the above

e)

none of the above

72.

The purpose of the iodine is to do which of the following?

a)

force crystal violet into the cell

b)

bind up crystal violet

c)

force malachite green into the cell

d)

bind up malachite green

73.

What is an agar that is Mueller Hinton agar with 5% chocolate sheep blood and antibiotics used for isolating pathogenic neisseria bacteria (inhibits the growth of most other microorganisms)?

a)

nutrient agar

b)

blood agar

c)

ssa agar

d)

chocolate agar

e)

thayer-martin chocolate agar

74.

Another name for spiral- or helical-shaped bacteria is:

a)

coccus

b)

bacillus

c)

coccobacillus

d)

spirochete

e)

none of the above

75.

The purpose of the steam is to do which of the following?

a)

force crystal violet into the cell

b)

bind up crystal violet

c)

force malachite green into the cell

d)

bind up malachite green

76.

What is an agar that is used for determining if the organism is halophilic and indicates if it can ferment mannitol?

a)

MAC

b)

MSA

c)

EMB

d)

Mueller Hinton agar

e)

Thayer-Martin Chocolate Agar

77.

______ is used to determine whether a bacteria can anerobically metabolize a specific sugar and whether is produces gas as a byproduct of its metabolism.

a)

catalase test

b)

growing in nutrient broth

c)

carbohydrate fermentation test

d)

EMB/MAC

78.

What is an agar that is made from heat-treated blood (which turns brown) grows fastidious (particular) respiratory bacteria?

a)

nutrient agar

b)

blood agar

c)

ssa agar

d)

chocolate agar

e)

thayer-martin chocolate agar

79.

What is an agar that causes bacteria to change color if they ferment lactose (E. coli bacteria turn green)?

a)

agar

b)

eosin methylene blue agar

c)

thioglycollate broth

d)

macconkey agar

80.

This bacterial growth curve is used to show on bacteria grow in liquid culture or on a bacterial growth plate. Which is the best place to collect healthy bacteria for cold storage?

a)

a

b)

b

c)

c

d)

d

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