single-stranded DNA "overhangs" that can bind to complementary DNA

double stranded DNA aligned with each other

single-stranded DNA aligned with host DNA

ends of a double stranded DNA strand

First letter in Cap for Genus, next two letter in small for species, next one letter for strain and roman numeral for order of discovery

First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and English numeral for order of discovery

First two letter in Cap for Genus, next one letter in capital for species, next one letter for strain and roman numeral for order of discovery

First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and roman numeral for order of discovery

Alul

HaeIII

BamHI

HindIII

EcoRI

Alul

HaeIII

BamHI

HindIII

EcoRI

defense by protozoa against antibiotics

tools by viruses to cleave bacterial genome

defense by bacteria against bacteriophages viruses

tools to cleave DNA during replication

A sticky end resulting from a restriction enzyme

A blunt end resulting from a restriction enzyme

A sequence that reads the same forwards and backwards

A sequence that is complementary to the other

pUC19

pBR322

pUC119

pUC18

a radium labelled marker

a type of sequence

resistant gene

a type of protein

Differentiate between transformant and non transformant

Differentiate between clones with vector plasmids and without vector plasmids

Differentiate between competent cells and non competent cells

Differntiate between negative and false negative

place of origin

sequence of genome where replication starts

where DNA are formed

where RNA are formed

Procedure though which a piece of protein incorporated into bacterium

Procedure though which a piece of lipid incorporated into bacterium

Procedure though which a piece of DNA incorporated into bacterium

Procedure though which a piece of carbohydrate incorporated into bacterium

code for the same gene product.

be cut by the same restriction enzyme.

originate from the same type of cell.

have identical sequences.

LacZ gene , X-gal

α-galactosidase and X-gal

Amp R gene and X-gal

β-galactosidase and X-gal

Insertional inactivation of β-galactosidase gene in recombinant bacteria

Inactivation of glycosidase gene in recombinant bacteria

Incompetence of recombinant bacteria containing β-galactosidase

insertional inactivation of α-galactosidase gene in non-recombinant bacteria

To increase the phage genomic DNA size

To reduce the phage genomic RNA size

To fit in other phage DNA

To fit in the foreign gene to be cloned

giving them a freeze shock

giving them a heat shock

giving them electrical current

all of above

to make cosmids infectious

to make cosmids competent

allow DNA to be packaged inside lambda phage heads

permit replication as plasmid in bacteria

Use ligase enzyme

Use alkaline phosphatase enzyme

Use restriction enzymes

Use polymerase enzyme

a).Watson and Crick

b).Arber ,Natha, Linn

c).Jacob and Monod

d).Beadle and Tatum

a).HindII

b). EcoRI

c).BamHI

d).HindIII

a).Peptidase

b).Amylases

c).Lipases

d).Nucleases

CGAAGC

GCTTCG

CGUUCG

GCUUCG

reform electrostatic attractions

reform hydrogen bonds

reform the sugar-phosphate bonds

reform OH bonds

Technique to identify desirable gene

Technique to recognise non-recombinant cells

Technique to recognise recombinant cells

Technique to recognise specific cell types

kanamycin

streptomycin

tetracycline

chloramphenicol

anywhere in the vector

a unique restriction site called MCS

outside lacZ gene

None of the above

Restriction-Modification system

Restriction-Modifying system

Retraction-Modifying system

Refraction-Modification system

the hybrid of a phage and a phagemid

have the ability to replicate as a plasmid

have the ability to replicate as a phage

none of above

BAC capacity is 300kb

YAC capacity is 1000kb

HAC capacity is 20Mb

Cosmid capacity is 25kb