WorksheetsTA Session 1 (2/7)
Total questions: 25
Worksheet time: 13mins
How many different amino acid side chains are there?
10
15
20
25
What kind of bond links amino acids to form proteins?
Ionic
Phosphodiester
Hydrogen
Peptide
Nucleotides are made up of...
Nitrogenous base + sugar
Nitrogenous base + sugar + phosphate
Sugar + phosphate
Which of the following has one (1) phosphate group?
AMP
ADP
ATP
UDP
Pyrimidines have ___ ring(s) and purines have ___ ring(s).
2, 1
1, 2
2, 0
0, 1
In DNA, the nucleotide pairs are ...
A-T and G-C
A-G and T-C
A-C and U-G
A-U and G-C
Gel electrophoresis separates DNA fragments by their ...
Size
Charge
Nucleotide content
Heterochromatin is ___, while euchromatin is ___.
decondensed, highly condensed
highly condensed, decondensed
double stranded, single stranded
single stranded, double stranded
Which histone modification makes histones more negatively charged, loosening chromatin?
Methylation
Acetylation
Restriction endonucleases used in molecular biology are...
Highly specific mammalian enzymes
Nonspecific mammalian enzymes
Highly specific bacterial enzymes
Nonspecific bacterial enzymes
DNA replication occurs during which stage of the cell cycle?
M phase
G1 phase
S phase
G2 phase
Meselson and Stahl used 14N- and 15N-containing DNA and centrifugation to show that DNA replication is ...
Fully conservative
Semi-conservative
Dispersive
Origins of replication (where DNA synthesis begins) tend to have more ....
A-T pairs
G-C pairs
_____ bonds form between the 3'OH of the growing DNA strand and the 5'phosphate of the incoming nucleotide.
Peptide
Ionic
Phosphodiester
Hydrogen
DNA polymerase cannot begin synthesis without an existing 3'OH group, so ___ generates a short RNA primer.
Primase
Helicase
Ligase
Nuclease
The ____ strand requires multiple RNA primers, rather than just one.
Leading
Lagging
This piece of replication machinery creates single-strand nicks in the DNA upstream of the replication fork to relieve torsional stress.
Helicase
Sliding Clamp
Topoisomeriase
Primase
____ has a built-in RNA primer AND a DNA synthesis ability that it uses to extend the end of the chromosome to ensure full replication in the lagging strand.
Primase
DNA polymerase
Topoisomerase
Telomerase
Which type of spotaneous damage results in a cytosine being chemically altered into a uracil?
Depurination
Deamination
Thymine-dimer formation
Mismatch
Double-stranded DNA breaks can be fixed in two ways.
Which way uses a template to re-synthesize the lost DNA, resulting in no nucleotide loss?
Non-homologous end-joining.
Homologous recombination.
Alpha helices and beta sheets are formed and held together by covalent bonds.
True
False
DNA contains ____, while RNA contains ____.
galactose, lactose
lactose, glucose
ribose, deoxyribose
deoxyribose, ribose
Which of the following does NOT describe plasmids?
Circular
Have replication origins that allow them to replicate in bacteria
Single-stranded
Introduced to bacteria by transformation
Nucleosomes are made up of all the following EXCEPT...
147 base pairs of DNA
2 molecules of H2A
2 molecules of H1
2 molecules of H3
The purpose of single-stranded DNA binding proteins is ...
To prevent the double helix from re-forming during replication
To promote the expression of a gene by recruiting RNA pol II
To load the sliding clamp onto the DNA behind DNA polymerase
