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Worksheets

TA Session 1 (2/7)

Total questions: 25

Worksheet time: 13mins

Name
Class
Date
1.

How many different amino acid side chains are there?

a)

10

b)

15

c)

20

d)

25

2.

What kind of bond links amino acids to form proteins?

a)

Ionic

b)

Phosphodiester

c)

Hydrogen

d)

Peptide

3.

Nucleotides are made up of...

a)

Nitrogenous base + sugar

b)

Nitrogenous base + sugar + phosphate

c)

Sugar + phosphate

4.

Which of the following has one (1) phosphate group?

a)

AMP

b)

ADP

c)

ATP

d)

UDP

5.

Pyrimidines have ___ ring(s) and purines have ___ ring(s).

a)

2, 1

b)

1, 2

c)

2, 0

d)

0, 1

6.

In DNA, the nucleotide pairs are ...

a)

A-T and G-C

b)

A-G and T-C

c)

A-C and U-G

d)

A-U and G-C

7.

Gel electrophoresis separates DNA fragments by their ...

a)

Size

b)

Charge

c)

Nucleotide content

8.

Heterochromatin is ___, while euchromatin is ___.

a)

decondensed, highly condensed

b)

highly condensed, decondensed

c)

double stranded, single stranded

d)

single stranded, double stranded

9.

Which histone modification makes histones more negatively charged, loosening chromatin?

a)

Methylation

b)

Acetylation

10.

Restriction endonucleases used in molecular biology are...

a)

Highly specific mammalian enzymes

b)

Nonspecific mammalian enzymes

c)

Highly specific bacterial enzymes

d)

Nonspecific bacterial enzymes

11.

DNA replication occurs during which stage of the cell cycle?

a)

M phase

b)

G1 phase

c)

S phase

d)

G2 phase

12.

Meselson and Stahl used 14N- and 15N-containing DNA and centrifugation to show that DNA replication is ...

a)

Fully conservative

b)

Semi-conservative

c)

Dispersive

13.

Origins of replication (where DNA synthesis begins) tend to have more ....

a)

A-T pairs

b)

G-C pairs

14.

_____ bonds form between the 3'OH of the growing DNA strand and the 5'phosphate of the incoming nucleotide.

a)

Peptide

b)

Ionic

c)

Phosphodiester

d)

Hydrogen

15.

DNA polymerase cannot begin synthesis without an existing 3'OH group, so ___ generates a short RNA primer.

a)

Primase

b)

Helicase

c)

Ligase

d)

Nuclease

16.

The ____ strand requires multiple RNA primers, rather than just one.

a)

Leading

b)

Lagging

17.

This piece of replication machinery creates single-strand nicks in the DNA upstream of the replication fork to relieve torsional stress.

a)

Helicase

b)

Sliding Clamp

c)

Topoisomeriase

d)

Primase

18.

____ has a built-in RNA primer AND a DNA synthesis ability that it uses to extend the end of the chromosome to ensure full replication in the lagging strand.

a)

Primase

b)

DNA polymerase

c)

Topoisomerase

d)

Telomerase

19.

Which type of spotaneous damage results in a cytosine being chemically altered into a uracil?

a)

Depurination

b)

Deamination

c)

Thymine-dimer formation

d)

Mismatch

20.

Double-stranded DNA breaks can be fixed in two ways.

Which way uses a template to re-synthesize the lost DNA, resulting in no nucleotide loss?

a)

Non-homologous end-joining.

b)

Homologous recombination.

21.

Alpha helices and beta sheets are formed and held together by covalent bonds.

a)

True

b)

False

22.

DNA contains ____, while RNA contains ____.

a)

galactose, lactose

b)

lactose, glucose

c)

ribose, deoxyribose

d)

deoxyribose, ribose

23.

Which of the following does NOT describe plasmids?

a)

Circular

b)

Have replication origins that allow them to replicate in bacteria

c)

Single-stranded

d)

Introduced to bacteria by transformation

24.

Nucleosomes are made up of all the following EXCEPT...

a)

147 base pairs of DNA

b)

2 molecules of H2A

c)

2 molecules of H1

d)

2 molecules of H3

25.

The purpose of single-stranded DNA binding proteins is ...

a)

To prevent the double helix from re-forming during replication

b)

To promote the expression of a gene by recruiting RNA pol II

c)

To load the sliding clamp onto the DNA behind DNA polymerase